Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2224 | 3' | -53 | NC_001405.1 | + | 10336 | 0.66 | 0.692712 |
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Target: 5'- cGGCGGCGGcugGCGGuAGAGGGgccagcguAGGg -3' miRNA: 3'- uCUGUCGCUa--CGCCuUCUCUCac------UCC- -5' |
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| 2224 | 3' | -53 | NC_001405.1 | + | 13967 | 0.67 | 0.666482 |
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Target: 5'- cGGCAGCGAcaGCagcguccuggauuuGGGAGGGAGUGGc- -3' miRNA: 3'- uCUGUCGCUa-CG--------------CCUUCUCUCACUcc -5' |
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| 2224 | 3' | -53 | NC_001405.1 | + | 31020 | 0.69 | 0.544208 |
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Target: 5'- aAGAUAGUgGGUGCGGAGGgacaAGAacaUGAGGa -3' miRNA: 3'- -UCUGUCG-CUACGCCUUC----UCUc--ACUCC- -5' |
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| 2224 | 3' | -53 | NC_001405.1 | + | 954 | 0.71 | 0.407377 |
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Target: 5'- uGACGaCGAgGauGAAGAGGGUGAGGa -3' miRNA: 3'- uCUGUcGCUaCgcCUUCUCUCACUCC- -5' |
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| 2224 | 3' | -53 | NC_001405.1 | + | 15074 | 0.72 | 0.388273 |
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Target: 5'- uGGCAGCGgcGCGGAAGAGAa----- -3' miRNA: 3'- uCUGUCGCuaCGCCUUCUCUcacucc -5' |
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| 2224 | 3' | -53 | NC_001405.1 | + | 9389 | 0.74 | 0.286561 |
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Target: 5'- uGGCGGCGGUGgGGGAGGGGG-GAc- -3' miRNA: 3'- uCUGUCGCUACgCCUUCUCUCaCUcc -5' |
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| 2224 | 3' | -53 | NC_001405.1 | + | 6090 | 1.11 | 0.00069 |
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Target: 5'- cAGACAGCGAUGCGGAAGAGAGUGAGGa -3' miRNA: 3'- -UCUGUCGCUACGCCUUCUCUCACUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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