Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22241 | 3' | -55.9 | NC_005045.1 | + | 3270 | 0.67 | 0.512675 |
Target: 5'- -aCCCAGGUaGCgguCGCUGuaguUCACCAg -3' miRNA: 3'- caGGGUUCGaCGau-GCGGCu---AGUGGU- -5' |
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22241 | 3' | -55.9 | NC_005045.1 | + | 4463 | 1.08 | 0.000597 |
Target: 5'- uGUCCCAAGCUGCUACGCCGAUCACCAg -3' miRNA: 3'- -CAGGGUUCGACGAUGCGGCUAGUGGU- -5' |
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22241 | 3' | -55.9 | NC_005045.1 | + | 4682 | 0.67 | 0.501967 |
Target: 5'- --aCCAccGCUGCUACcCCGggCGCCAa -3' miRNA: 3'- cagGGUu-CGACGAUGcGGCuaGUGGU- -5' |
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22241 | 3' | -55.9 | NC_005045.1 | + | 5026 | 0.66 | 0.545328 |
Target: 5'- cGUCC--AGCaGCUugGCCGGUgCGCUg -3' miRNA: 3'- -CAGGguUCGaCGAugCGGCUA-GUGGu -5' |
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22241 | 3' | -55.9 | NC_005045.1 | + | 6025 | 0.66 | 0.567466 |
Target: 5'- gGUCCgCAAGCUuccguacccgGCUGCGCagCGGUCcguCCAu -3' miRNA: 3'- -CAGG-GUUCGA----------CGAUGCG--GCUAGu--GGU- -5' |
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22241 | 3' | -55.9 | NC_005045.1 | + | 7727 | 0.67 | 0.534362 |
Target: 5'- aUCCCAAGUggguggGCUACGgguauccugccCCGgAUUACCAu -3' miRNA: 3'- cAGGGUUCGa-----CGAUGC-----------GGC-UAGUGGU- -5' |
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22241 | 3' | -55.9 | NC_005045.1 | + | 15859 | 0.68 | 0.43009 |
Target: 5'- --gCCGAGgUGCUguggauGCGCCGAaCGCCGa -3' miRNA: 3'- cagGGUUCgACGA------UGCGGCUaGUGGU- -5' |
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22241 | 3' | -55.9 | NC_005045.1 | + | 18783 | 0.66 | 0.601065 |
Target: 5'- --aCCAuguGCUGgUACGCCGcaccaagCACCAg -3' miRNA: 3'- cagGGUu--CGACgAUGCGGCua-----GUGGU- -5' |
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22241 | 3' | -55.9 | NC_005045.1 | + | 19430 | 0.68 | 0.460184 |
Target: 5'- -aCCCAGGCUGCccgguCGUCGAaguaCACCu -3' miRNA: 3'- caGGGUUCGACGau---GCGGCUa---GUGGu -5' |
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22241 | 3' | -55.9 | NC_005045.1 | + | 23196 | 0.71 | 0.322261 |
Target: 5'- -cCCCcGGCUGCcGCGCCGAccccgaACCAu -3' miRNA: 3'- caGGGuUCGACGaUGCGGCUag----UGGU- -5' |
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22241 | 3' | -55.9 | NC_005045.1 | + | 23311 | 0.66 | 0.578622 |
Target: 5'- -aCCgGAGcCUGCUGC-UgGAUCACCGg -3' miRNA: 3'- caGGgUUC-GACGAUGcGgCUAGUGGU- -5' |
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22241 | 3' | -55.9 | NC_005045.1 | + | 25004 | 0.68 | 0.439994 |
Target: 5'- -gCCCAAGUg---GCGCCGGUCcagGCCAa -3' miRNA: 3'- caGGGUUCGacgaUGCGGCUAG---UGGU- -5' |
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22241 | 3' | -55.9 | NC_005045.1 | + | 27817 | 0.73 | 0.2297 |
Target: 5'- gGUCCCAAuGCUGCUugGUCagcuccagcUCACCAg -3' miRNA: 3'- -CAGGGUU-CGACGAugCGGcu-------AGUGGU- -5' |
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22241 | 3' | -55.9 | NC_005045.1 | + | 29372 | 0.68 | 0.439994 |
Target: 5'- -gCCUucuGCUGCUGCGCCag-CGCCu -3' miRNA: 3'- caGGGuu-CGACGAUGCGGcuaGUGGu -5' |
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22241 | 3' | -55.9 | NC_005045.1 | + | 29530 | 0.66 | 0.553047 |
Target: 5'- cUUCCAGGCUgaGCUGCcgggacaguucgucGCCGGUgGCCu -3' miRNA: 3'- cAGGGUUCGA--CGAUG--------------CGGCUAgUGGu -5' |
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22241 | 3' | -55.9 | NC_005045.1 | + | 30518 | 0.66 | 0.567466 |
Target: 5'- -gCUCAGGUcGCUuuCGCCGGUCAgCAu -3' miRNA: 3'- caGGGUUCGaCGAu-GCGGCUAGUgGU- -5' |
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22241 | 3' | -55.9 | NC_005045.1 | + | 33611 | 0.71 | 0.306376 |
Target: 5'- gGUCacgcggGAGCUGCUGCGCCGGg-ACCGg -3' miRNA: 3'- -CAGgg----UUCGACGAUGCGGCUagUGGU- -5' |
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22241 | 3' | -55.9 | NC_005045.1 | + | 36861 | 0.68 | 0.450027 |
Target: 5'- cGUCCCuAGCggagGCUGgacgaacauguuCGCCGG-CACCGg -3' miRNA: 3'- -CAGGGuUCGa---CGAU------------GCGGCUaGUGGU- -5' |
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22241 | 3' | -55.9 | NC_005045.1 | + | 38063 | 0.67 | 0.491359 |
Target: 5'- -gCCCAGGC-GCUGCccucuggccGCCGAUCGuCUAg -3' miRNA: 3'- caGGGUUCGaCGAUG---------CGGCUAGU-GGU- -5' |
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22241 | 3' | -55.9 | NC_005045.1 | + | 39559 | 0.7 | 0.364623 |
Target: 5'- -gCCCGAGCUGUaccugccgGCGCUGAUgGCUg -3' miRNA: 3'- caGGGUUCGACGa-------UGCGGCUAgUGGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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