miRNA display CGI


Results 21 - 24 of 24 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22243 3' -59 NC_005045.1 + 24407 0.7 0.249198
Target:  5'- gGCACGaCgGCCcuugGCCUcgacgUUGCCCAGG-CGa -3'
miRNA:   3'- -UGUGC-GgCGG----UGGA-----AACGGGUCCaGC- -5'
22243 3' -59 NC_005045.1 + 12777 0.7 0.236624
Target:  5'- uCACGCgGCUGCCg--GCuuCCGGGUCGu -3'
miRNA:   3'- uGUGCGgCGGUGGaaaCG--GGUCCAGC- -5'
22243 3' -59 NC_005045.1 + 18823 0.71 0.20423
Target:  5'- gGCACGgcaggcgcagcuCCGCCACCUgggccaggacaagaUGCCCAaGGUCu -3'
miRNA:   3'- -UGUGC------------GGCGGUGGAa-------------ACGGGU-CCAGc -5'
22243 3' -59 NC_005045.1 + 6545 0.93 0.004936
Target:  5'- cACACGCCGCCACCUUguCCCAGGUCGc -3'
miRNA:   3'- -UGUGCGGCGGUGGAAacGGGUCCAGC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.