Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22243 | 3' | -59 | NC_005045.1 | + | 24407 | 0.7 | 0.249198 |
Target: 5'- gGCACGaCgGCCcuugGCCUcgacgUUGCCCAGG-CGa -3' miRNA: 3'- -UGUGC-GgCGG----UGGA-----AACGGGUCCaGC- -5' |
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22243 | 3' | -59 | NC_005045.1 | + | 12777 | 0.7 | 0.236624 |
Target: 5'- uCACGCgGCUGCCg--GCuuCCGGGUCGu -3' miRNA: 3'- uGUGCGgCGGUGGaaaCG--GGUCCAGC- -5' |
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22243 | 3' | -59 | NC_005045.1 | + | 18823 | 0.71 | 0.20423 |
Target: 5'- gGCACGgcaggcgcagcuCCGCCACCUgggccaggacaagaUGCCCAaGGUCu -3' miRNA: 3'- -UGUGC------------GGCGGUGGAa-------------ACGGGU-CCAGc -5' |
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22243 | 3' | -59 | NC_005045.1 | + | 6545 | 0.93 | 0.004936 |
Target: 5'- cACACGCCGCCACCUUguCCCAGGUCGc -3' miRNA: 3'- -UGUGCGGCGGUGGAAacGGGUCCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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