Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22245 | 5' | -58 | NC_005045.1 | + | 8664 | 1.07 | 0.000405 |
Target: 5'- gGAGGCCCUUCAUGUACGCCGGCAGCAc -3' miRNA: 3'- -CUCCGGGAAGUACAUGCGGCCGUCGU- -5' |
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22245 | 5' | -58 | NC_005045.1 | + | 39338 | 0.75 | 0.118464 |
Target: 5'- cGAGGCCCUUgCcgGUGCGCgGGUuguGGCc -3' miRNA: 3'- -CUCCGGGAA-GuaCAUGCGgCCG---UCGu -5' |
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22245 | 5' | -58 | NC_005045.1 | + | 23875 | 0.73 | 0.166019 |
Target: 5'- aGGGUCCg-CGUGUugguugaagACGCCGGCAGCc -3' miRNA: 3'- cUCCGGGaaGUACA---------UGCGGCCGUCGu -5' |
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22245 | 5' | -58 | NC_005045.1 | + | 13624 | 0.69 | 0.290769 |
Target: 5'- cAGGCgCUcgcUCAUGUA-GCgGGCAGCGu -3' miRNA: 3'- cUCCGgGA---AGUACAUgCGgCCGUCGU- -5' |
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22245 | 5' | -58 | NC_005045.1 | + | 20980 | 0.68 | 0.35387 |
Target: 5'- cAGGCCCaUCAguucggaUGggGCGCCGGCcuuuGCGu -3' miRNA: 3'- cUCCGGGaAGU-------ACa-UGCGGCCGu---CGU- -5' |
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22245 | 5' | -58 | NC_005045.1 | + | 26821 | 0.66 | 0.427986 |
Target: 5'- cGGGGCCUUg-GUGga-GCCGGUGGCc -3' miRNA: 3'- -CUCCGGGAagUACaugCGGCCGUCGu -5' |
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22245 | 5' | -58 | NC_005045.1 | + | 21820 | 0.66 | 0.437756 |
Target: 5'- cGAGGUUgUUCAcuagcaggGCGCCGGCAGa- -3' miRNA: 3'- -CUCCGGgAAGUaca-----UGCGGCCGUCgu -5' |
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22245 | 5' | -58 | NC_005045.1 | + | 11789 | 0.71 | 0.229409 |
Target: 5'- cAGGCCCguggCAUGUGgGCUGGCuaccuggAGCGc -3' miRNA: 3'- cUCCGGGaa--GUACAUgCGGCCG-------UCGU- -5' |
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22245 | 5' | -58 | NC_005045.1 | + | 34851 | 0.7 | 0.242524 |
Target: 5'- -cGGCCCUUCGaGUGCuCCaGCAGUAc -3' miRNA: 3'- cuCCGGGAAGUaCAUGcGGcCGUCGU- -5' |
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22245 | 5' | -58 | NC_005045.1 | + | 6903 | 0.69 | 0.31364 |
Target: 5'- aAGGCCCUgugCGUugACGaCCGGCAcaGCAg -3' miRNA: 3'- cUCCGGGAa--GUAcaUGC-GGCCGU--CGU- -5' |
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22245 | 5' | -58 | NC_005045.1 | + | 23272 | 0.68 | 0.329631 |
Target: 5'- --aGCCCggUCAgguggGUGCGCCG-CAGCAa -3' miRNA: 3'- cucCGGGa-AGUa----CAUGCGGCcGUCGU- -5' |
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22245 | 5' | -58 | NC_005045.1 | + | 21061 | 0.67 | 0.381143 |
Target: 5'- -uGGCCa-----GUugGCCGGCGGCGc -3' miRNA: 3'- cuCCGGgaaguaCAugCGGCCGUCGU- -5' |
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22245 | 5' | -58 | NC_005045.1 | + | 36871 | 0.67 | 0.418346 |
Target: 5'- gGAGGCUggaCgaaCAUGUuCGCCGGCAccgGCAa -3' miRNA: 3'- -CUCCGG---Gaa-GUACAuGCGGCCGU---CGU- -5' |
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22245 | 5' | -58 | NC_005045.1 | + | 26356 | 0.66 | 0.436773 |
Target: 5'- -cGGCCaCUUaCGUG-ACGCCGGacaacgcCAGCAc -3' miRNA: 3'- cuCCGG-GAA-GUACaUGCGGCC-------GUCGU- -5' |
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22245 | 5' | -58 | NC_005045.1 | + | 34263 | 0.66 | 0.437756 |
Target: 5'- -uGGCCCgcgCAgGUAUGCCGGgGGa- -3' miRNA: 3'- cuCCGGGaa-GUaCAUGCGGCCgUCgu -5' |
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22245 | 5' | -58 | NC_005045.1 | + | 29963 | 0.66 | 0.478065 |
Target: 5'- uGAGGCCCUggagcaGUGggacaagAUGCaGGCGGCc -3' miRNA: 3'- -CUCCGGGAag----UACa------UGCGgCCGUCGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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