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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22246 | 3' | -50.7 | NC_005045.1 | + | 32523 | 0.66 | 0.880472 |
|
Target: 5'- --aCGUCGCGg-GAUGGCCgguauCUCGGc -3' miRNA: 3'- gaaGUAGCGCagUUACUGGac---GAGCC- -5' |
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| 22246 | 3' | -50.7 | NC_005045.1 | + | 30840 | 0.66 | 0.872402 |
|
Target: 5'- cCUUCGacaUGCGgcu-UGugCUGCUCGGc -3' miRNA: 3'- -GAAGUa--GCGCaguuACugGACGAGCC- -5' |
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| 22246 | 3' | -50.7 | NC_005045.1 | + | 20333 | 0.66 | 0.89577 |
|
Target: 5'- gUUCAUUGCcauGUaCAGUGGCCccugcgUGCUCGu -3' miRNA: 3'- gAAGUAGCG---CA-GUUACUGG------ACGAGCc -5' |
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| 22246 | 3' | -50.7 | NC_005045.1 | + | 19547 | 0.67 | 0.846595 |
|
Target: 5'- gCUUCAUCGUccacaUgGAUGcaACCUGCUCaGGa -3' miRNA: 3'- -GAAGUAGCGc----AgUUAC--UGGACGAG-CC- -5' |
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| 22246 | 3' | -50.7 | NC_005045.1 | + | 14471 | 0.7 | 0.701966 |
|
Target: 5'- gUUCAUCuuGUCGAaGGCCaGCUUGGu -3' miRNA: 3'- gAAGUAGcgCAGUUaCUGGaCGAGCC- -5' |
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| 22246 | 3' | -50.7 | NC_005045.1 | + | 9003 | 1.11 | 0.00188 |
|
Target: 5'- gCUUCAUCGCGUCAAUGACCUGCUCGGc -3' miRNA: 3'- -GAAGUAGCGCAGUUACUGGACGAGCC- -5' |
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| 22246 | 3' | -50.7 | NC_005045.1 | + | 6269 | 0.68 | 0.798824 |
|
Target: 5'- --gCGUCGUGggCAGUGugcugacguACCUGCUCGu -3' miRNA: 3'- gaaGUAGCGCa-GUUAC---------UGGACGAGCc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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