Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22248 | 5' | -59.8 | NC_005045.1 | + | 41994 | 0.67 | 0.348687 |
Target: 5'- --gGCUGGGUugGCUGGugUgGGCgGg -3' miRNA: 3'- ggaCGACCCAugUGGUCugGgCCGgU- -5' |
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22248 | 5' | -59.8 | NC_005045.1 | + | 39246 | 0.74 | 0.113242 |
Target: 5'- gCCUGCUcguccaGGGUcgggaACCGG-CCCGGCCAg -3' miRNA: 3'- -GGACGA------CCCAug---UGGUCuGGGCCGGU- -5' |
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22248 | 5' | -59.8 | NC_005045.1 | + | 38842 | 0.68 | 0.327684 |
Target: 5'- aCCUGCgccccgaggcccgcaUGGGUG-ACCGuACCuCGGCCAc -3' miRNA: 3'- -GGACG---------------ACCCAUgUGGUcUGG-GCCGGU- -5' |
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22248 | 5' | -59.8 | NC_005045.1 | + | 38706 | 0.67 | 0.374092 |
Target: 5'- gCCUGUaucuauguggUGuGGUGCAUcacgCAGaACCCGGCUAc -3' miRNA: 3'- -GGACG----------AC-CCAUGUG----GUC-UGGGCCGGU- -5' |
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22248 | 5' | -59.8 | NC_005045.1 | + | 37327 | 0.69 | 0.273108 |
Target: 5'- gUCUGUcgUGGcgcgcuaucaGUGCGCCGGACaauaaCGGCCAg -3' miRNA: 3'- -GGACG--ACC----------CAUGUGGUCUGg----GCCGGU- -5' |
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22248 | 5' | -59.8 | NC_005045.1 | + | 36958 | 0.66 | 0.391712 |
Target: 5'- aCCg---GGGUACgguuGCCGGugCCGGCgAa -3' miRNA: 3'- -GGacgaCCCAUG----UGGUCugGGCCGgU- -5' |
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22248 | 5' | -59.8 | NC_005045.1 | + | 36245 | 0.7 | 0.222571 |
Target: 5'- uUCUGCaagcgguugaUGGucACGCCAGGgCCGGCCAc -3' miRNA: 3'- -GGACG----------ACCcaUGUGGUCUgGGCCGGU- -5' |
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22248 | 5' | -59.8 | NC_005045.1 | + | 33204 | 0.71 | 0.200421 |
Target: 5'- gCCaUGCcGGGuUACACCaAGGCUCaGGCCAa -3' miRNA: 3'- -GG-ACGaCCC-AUGUGG-UCUGGG-CCGGU- -5' |
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22248 | 5' | -59.8 | NC_005045.1 | + | 32046 | 0.69 | 0.24677 |
Target: 5'- --gGCU-GGUAUGCUGGACCCGGCa- -3' miRNA: 3'- ggaCGAcCCAUGUGGUCUGGGCCGgu -5' |
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22248 | 5' | -59.8 | NC_005045.1 | + | 31388 | 0.66 | 0.404363 |
Target: 5'- aCUGCcGGGcACGCCcuucgcccaucuggaAGGCCauacCGGCCAa -3' miRNA: 3'- gGACGaCCCaUGUGG---------------UCUGG----GCCGGU- -5' |
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22248 | 5' | -59.8 | NC_005045.1 | + | 30025 | 0.67 | 0.365485 |
Target: 5'- uCgUGCaGGGUACGCa--GCUCGGCCu -3' miRNA: 3'- -GgACGaCCCAUGUGgucUGGGCCGGu -5' |
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22248 | 5' | -59.8 | NC_005045.1 | + | 29463 | 0.66 | 0.428529 |
Target: 5'- aCUGUuacccUGGGUACggaaGCCAcgggcGGCCUGGUCGa -3' miRNA: 3'- gGACG-----ACCCAUG----UGGU-----CUGGGCCGGU- -5' |
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22248 | 5' | -59.8 | NC_005045.1 | + | 27891 | 0.71 | 0.195192 |
Target: 5'- aCCgagaUGGGUACGCUccauguAGGCgCCGGCCAc -3' miRNA: 3'- -GGacg-ACCCAUGUGG------UCUG-GGCCGGU- -5' |
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22248 | 5' | -59.8 | NC_005045.1 | + | 27436 | 0.66 | 0.391712 |
Target: 5'- gCCgGCUGaGUACAuCCAGGCggCGGCCu -3' miRNA: 3'- -GGaCGACcCAUGU-GGUCUGg-GCCGGu -5' |
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22248 | 5' | -59.8 | NC_005045.1 | + | 25972 | 0.72 | 0.153291 |
Target: 5'- aCCUGCUGcaUACcgACCAGcCCUGGCCGa -3' miRNA: 3'- -GGACGACccAUG--UGGUCuGGGCCGGU- -5' |
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22248 | 5' | -59.8 | NC_005045.1 | + | 25412 | 0.66 | 0.409863 |
Target: 5'- aCCUGgUGGaGgcacaagcugACACCuGACCCcacgGGCCGc -3' miRNA: 3'- -GGACgACC-Ca---------UGUGGuCUGGG----CCGGU- -5' |
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22248 | 5' | -59.8 | NC_005045.1 | + | 23343 | 0.66 | 0.391712 |
Target: 5'- gUUGCUGcGGcGCACCcaccuGACCgGGCUg -3' miRNA: 3'- gGACGAC-CCaUGUGGu----CUGGgCCGGu -5' |
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22248 | 5' | -59.8 | NC_005045.1 | + | 23301 | 0.67 | 0.365485 |
Target: 5'- gCUGCUGGau-CACCGGGuuCGGCa- -3' miRNA: 3'- gGACGACCcauGUGGUCUggGCCGgu -5' |
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22248 | 5' | -59.8 | NC_005045.1 | + | 17366 | 0.68 | 0.301654 |
Target: 5'- --gGCUGGGUGCAUacgCAGACC-GGCa- -3' miRNA: 3'- ggaCGACCCAUGUG---GUCUGGgCCGgu -5' |
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22248 | 5' | -59.8 | NC_005045.1 | + | 14767 | 0.71 | 0.182151 |
Target: 5'- uCCUGCUGGGUgGCGgCgaGGACCUgaucaucaagccgaaGGCCAa -3' miRNA: 3'- -GGACGACCCA-UGUgG--UCUGGG---------------CCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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