Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22249 | 3' | -58.1 | NC_005045.1 | + | 11775 | 1.11 | 0.000249 |
Target: 5'- cGGGCCGGACAGGUACUCGCUCAGCAGg -3' miRNA: 3'- -CCCGGCCUGUCCAUGAGCGAGUCGUC- -5' |
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22249 | 3' | -58.1 | NC_005045.1 | + | 15978 | 0.8 | 0.059396 |
Target: 5'- aGGCCGGACgaAGGU-CUCgGCUUAGCAGg -3' miRNA: 3'- cCCGGCCUG--UCCAuGAG-CGAGUCGUC- -5' |
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22249 | 3' | -58.1 | NC_005045.1 | + | 4883 | 0.76 | 0.114699 |
Target: 5'- aGGCUGGACAGG----CGCUCGGCGGu -3' miRNA: 3'- cCCGGCCUGUCCaugaGCGAGUCGUC- -5' |
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22249 | 3' | -58.1 | NC_005045.1 | + | 23467 | 0.73 | 0.173706 |
Target: 5'- gGGGCCGGcugaGCGGGUGCUUGCggaaCAaCGGg -3' miRNA: 3'- -CCCGGCC----UGUCCAUGAGCGa---GUcGUC- -5' |
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22249 | 3' | -58.1 | NC_005045.1 | + | 16962 | 0.71 | 0.258318 |
Target: 5'- aGGGCCaccaGCAGGUGCaggUCGUcCAGCGGg -3' miRNA: 3'- -CCCGGcc--UGUCCAUG---AGCGaGUCGUC- -5' |
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22249 | 3' | -58.1 | NC_005045.1 | + | 16072 | 0.7 | 0.285958 |
Target: 5'- uGGGCCGaGCAGGgcaaacucUGCcCGCUCuGCGGc -3' miRNA: 3'- -CCCGGCcUGUCC--------AUGaGCGAGuCGUC- -5' |
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22249 | 3' | -58.1 | NC_005045.1 | + | 18592 | 0.7 | 0.30063 |
Target: 5'- -uGCCGaGgcGCAGGUAgUCGCcgUCGGCAGg -3' miRNA: 3'- ccCGGC-C--UGUCCAUgAGCG--AGUCGUC- -5' |
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22249 | 3' | -58.1 | NC_005045.1 | + | 38901 | 0.69 | 0.348099 |
Target: 5'- cGGGCCucggGGcGCAGGUA--CGC-CAGCAGg -3' miRNA: 3'- -CCCGG----CC-UGUCCAUgaGCGaGUCGUC- -5' |
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22249 | 3' | -58.1 | NC_005045.1 | + | 11574 | 0.68 | 0.365069 |
Target: 5'- aGGaaGGcCAGGUACUCGUcccggUAGCGGg -3' miRNA: 3'- cCCggCCuGUCCAUGAGCGa----GUCGUC- -5' |
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22249 | 3' | -58.1 | NC_005045.1 | + | 32624 | 0.68 | 0.373765 |
Target: 5'- aGGGCCugGGGCAGcGUACcgCGUUCcgugugguGCAGg -3' miRNA: 3'- -CCCGG--CCUGUC-CAUGa-GCGAGu-------CGUC- -5' |
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22249 | 3' | -58.1 | NC_005045.1 | + | 34474 | 0.68 | 0.382601 |
Target: 5'- gGGGCCGaGCuGGUGCUCaCgCGGCAc -3' miRNA: 3'- -CCCGGCcUGuCCAUGAGcGaGUCGUc -5' |
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22249 | 3' | -58.1 | NC_005045.1 | + | 5135 | 0.68 | 0.391575 |
Target: 5'- aGGGCCGGGCcuauuacgugcGGGccuUACUCGCccUgGGCGa -3' miRNA: 3'- -CCCGGCCUG-----------UCC---AUGAGCG--AgUCGUc -5' |
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22249 | 3' | -58.1 | NC_005045.1 | + | 41598 | 0.68 | 0.391575 |
Target: 5'- cGGGuCCGGugGGGUAgcgccgccCUCGCcgCccagGGCAGu -3' miRNA: 3'- -CCC-GGCCugUCCAU--------GAGCGa-G----UCGUC- -5' |
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22249 | 3' | -58.1 | NC_005045.1 | + | 22478 | 0.67 | 0.428808 |
Target: 5'- -aGCuCGuACAGGccgaguuuCUCGCUCAGCAGg -3' miRNA: 3'- ccCG-GCcUGUCCau------GAGCGAGUCGUC- -5' |
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22249 | 3' | -58.1 | NC_005045.1 | + | 30238 | 0.67 | 0.438437 |
Target: 5'- aGGCCGaGCAGGgcgccgugGCcugggCGCUCaAGCAGa -3' miRNA: 3'- cCCGGCcUGUCCa-------UGa----GCGAG-UCGUC- -5' |
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22249 | 3' | -58.1 | NC_005045.1 | + | 26563 | 0.67 | 0.44819 |
Target: 5'- gGGGCgugcaGGACGGGUACaUCGCauuccgUGGCGa -3' miRNA: 3'- -CCCGg----CCUGUCCAUG-AGCGa-----GUCGUc -5' |
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22249 | 3' | -58.1 | NC_005045.1 | + | 16759 | 0.67 | 0.44819 |
Target: 5'- aGGGCUGGGu--GUGgUCGCUCuggaAGCAGa -3' miRNA: 3'- -CCCGGCCUgucCAUgAGCGAG----UCGUC- -5' |
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22249 | 3' | -58.1 | NC_005045.1 | + | 25050 | 0.67 | 0.44819 |
Target: 5'- uGGGCgGuGAUcaGGGU-CUUGCUCGGgAGg -3' miRNA: 3'- -CCCGgC-CUG--UCCAuGAGCGAGUCgUC- -5' |
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22249 | 3' | -58.1 | NC_005045.1 | + | 14035 | 0.66 | 0.457069 |
Target: 5'- aGGCCGGAgUAGGgaagGgUCGUcaggcguUCGGCAGc -3' miRNA: 3'- cCCGGCCU-GUCCa---UgAGCG-------AGUCGUC- -5' |
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22249 | 3' | -58.1 | NC_005045.1 | + | 6906 | 0.66 | 0.467044 |
Target: 5'- aGGCCGGACAGGUcgaaggcAC-CGagggcCAGCAc -3' miRNA: 3'- cCCGGCCUGUCCA-------UGaGCga---GUCGUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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