Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22249 | 3' | -58.1 | NC_005045.1 | + | 11574 | 0.68 | 0.365069 |
Target: 5'- aGGaaGGcCAGGUACUCGUcccggUAGCGGg -3' miRNA: 3'- cCCggCCuGUCCAUGAGCGa----GUCGUC- -5' |
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22249 | 3' | -58.1 | NC_005045.1 | + | 6906 | 0.66 | 0.467044 |
Target: 5'- aGGCCGGACAGGUcgaaggcAC-CGagggcCAGCAc -3' miRNA: 3'- cCCGGCCUGUCCA-------UGaGCga---GUCGUc -5' |
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22249 | 3' | -58.1 | NC_005045.1 | + | 5135 | 0.68 | 0.391575 |
Target: 5'- aGGGCCGGGCcuauuacgugcGGGccuUACUCGCccUgGGCGa -3' miRNA: 3'- -CCCGGCCUG-----------UCC---AUGAGCG--AgUCGUc -5' |
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22249 | 3' | -58.1 | NC_005045.1 | + | 4883 | 0.76 | 0.114699 |
Target: 5'- aGGCUGGACAGG----CGCUCGGCGGu -3' miRNA: 3'- cCCGGCCUGUCCaugaGCGAGUCGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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