Results 1 - 20 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22250 | 3' | -57.3 | NC_005045.1 | + | 20808 | 0.65 | 0.535329 |
Target: 5'- cGGUCGGCgagacguggugcgaGCAgUCCGcuugcgcCGCCGAGAc- -3' miRNA: 3'- -CCGGCCG--------------UGUgAGGUa------GCGGCUCUuu -5' |
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22250 | 3' | -57.3 | NC_005045.1 | + | 5836 | 0.66 | 0.527796 |
Target: 5'- cGUCGGCGCGCUUCG-CGUCGGc--- -3' miRNA: 3'- cCGGCCGUGUGAGGUaGCGGCUcuuu -5' |
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22250 | 3' | -57.3 | NC_005045.1 | + | 13780 | 0.66 | 0.517106 |
Target: 5'- uGGCaUGGCGCugUCCcau-CCGAGGGAa -3' miRNA: 3'- -CCG-GCCGUGugAGGuagcGGCUCUUU- -5' |
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22250 | 3' | -57.3 | NC_005045.1 | + | 3733 | 0.66 | 0.506503 |
Target: 5'- aGGCCgGGCAgauaGCgaCCAcgcCGCCGAGGAAc -3' miRNA: 3'- -CCGG-CCGUg---UGa-GGUa--GCGGCUCUUU- -5' |
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22250 | 3' | -57.3 | NC_005045.1 | + | 13221 | 0.66 | 0.505448 |
Target: 5'- gGGCCGGauggcaaCugGCgCCAguacCGCCGGGGAu -3' miRNA: 3'- -CCGGCC-------GugUGaGGUa---GCGGCUCUUu -5' |
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22250 | 3' | -57.3 | NC_005045.1 | + | 28675 | 0.66 | 0.500186 |
Target: 5'- aGCaGGCGCACUCCAUCcaggcucaggcuaagGCCGGc--- -3' miRNA: 3'- cCGgCCGUGUGAGGUAG---------------CGGCUcuuu -5' |
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22250 | 3' | -57.3 | NC_005045.1 | + | 4513 | 0.66 | 0.485585 |
Target: 5'- cGGgUGGCGCAUggUCC-UCGCCGAcaaGggGa -3' miRNA: 3'- -CCgGCCGUGUG--AGGuAGCGGCU---CuuU- -5' |
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22250 | 3' | -57.3 | NC_005045.1 | + | 4822 | 0.66 | 0.485585 |
Target: 5'- cGCCGaGCGcCugUCCAgccucgUCGCCG-GAGAc -3' miRNA: 3'- cCGGC-CGU-GugAGGU------AGCGGCuCUUU- -5' |
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22250 | 3' | -57.3 | NC_005045.1 | + | 18212 | 0.67 | 0.455008 |
Target: 5'- gGGCCGGCAgccaaGCUCCGgcacUUGCUGcGGAu -3' miRNA: 3'- -CCGGCCGUg----UGAGGU----AGCGGCuCUUu -5' |
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22250 | 3' | -57.3 | NC_005045.1 | + | 40064 | 0.67 | 0.445048 |
Target: 5'- uGCUGGCaggACACguggucggaaUCCAUCGCCGAu--- -3' miRNA: 3'- cCGGCCG---UGUG----------AGGUAGCGGCUcuuu -5' |
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22250 | 3' | -57.3 | NC_005045.1 | + | 18612 | 0.67 | 0.442084 |
Target: 5'- gGGgCGGCACGagcguccaccuggagCCGUCGCCG-GAGg -3' miRNA: 3'- -CCgGCCGUGUga-------------GGUAGCGGCuCUUu -5' |
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22250 | 3' | -57.3 | NC_005045.1 | + | 40331 | 0.67 | 0.435212 |
Target: 5'- cGGCCcuuGCgACGCUCCcggcccaUGCCGAGAGAc -3' miRNA: 3'- -CCGGc--CG-UGUGAGGua-----GCGGCUCUUU- -5' |
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22250 | 3' | -57.3 | NC_005045.1 | + | 12356 | 0.67 | 0.435212 |
Target: 5'- cGCUGGUcaucauccCACUUgGUCGCCGGGAu- -3' miRNA: 3'- cCGGCCGu-------GUGAGgUAGCGGCUCUuu -5' |
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22250 | 3' | -57.3 | NC_005045.1 | + | 34283 | 0.67 | 0.435212 |
Target: 5'- uGCCGGCGuaguuCUCCAUCaGCCGGucGAGGu -3' miRNA: 3'- cCGGCCGUgu---GAGGUAG-CGGCU--CUUU- -5' |
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22250 | 3' | -57.3 | NC_005045.1 | + | 22715 | 0.67 | 0.435212 |
Target: 5'- uGGgCGGCACauACUCgCucuUgGCCGAGAAc -3' miRNA: 3'- -CCgGCCGUG--UGAG-Gu--AgCGGCUCUUu -5' |
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22250 | 3' | -57.3 | NC_005045.1 | + | 28120 | 0.68 | 0.415923 |
Target: 5'- aGCCGGCA-ACUCaa-UGCCGGGGAGc -3' miRNA: 3'- cCGGCCGUgUGAGguaGCGGCUCUUU- -5' |
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22250 | 3' | -57.3 | NC_005045.1 | + | 8600 | 0.68 | 0.397166 |
Target: 5'- uGCCGGCguacaugaaggGC-CUCCA-CGCCGGGGc- -3' miRNA: 3'- cCGGCCG-----------UGuGAGGUaGCGGCUCUuu -5' |
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22250 | 3' | -57.3 | NC_005045.1 | + | 28975 | 0.69 | 0.361326 |
Target: 5'- aGUC-GCGCACUCCG-CGCCGAGc-- -3' miRNA: 3'- cCGGcCGUGUGAGGUaGCGGCUCuuu -5' |
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22250 | 3' | -57.3 | NC_005045.1 | + | 38437 | 0.69 | 0.352725 |
Target: 5'- cGCUGGaGgGCUUCAUCGCCGGuGAGAc -3' miRNA: 3'- cCGGCCgUgUGAGGUAGCGGCU-CUUU- -5' |
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22250 | 3' | -57.3 | NC_005045.1 | + | 35883 | 0.69 | 0.34427 |
Target: 5'- --aCGGCGUACUCgacgcgauccgCAUCGCCGAGGAAg -3' miRNA: 3'- ccgGCCGUGUGAG-----------GUAGCGGCUCUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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