Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22253 | 5' | -58 | NC_005045.1 | + | 40881 | 0.66 | 0.483704 |
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Target: 5'- -gGCgCGGCCuugguugaaGCGCUUGaaCGCUGUGCUGg -3' miRNA: 3'- uaUG-GUCGG---------CGUGAGC--GCGGCAUGAC- -5' |
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| 22253 | 5' | -58 | NC_005045.1 | + | 13322 | 0.66 | 0.483704 |
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Target: 5'- gGUGCCAcCCgGCGCUUGCcagaauccccgGCgGUACUGg -3' miRNA: 3'- -UAUGGUcGG-CGUGAGCG-----------CGgCAUGAC- -5' |
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| 22253 | 5' | -58 | NC_005045.1 | + | 6956 | 0.66 | 0.482669 |
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Target: 5'- gGUACgGGCCGCcgagcugGCUgGUGCCGagauCUGa -3' miRNA: 3'- -UAUGgUCGGCG-------UGAgCGCGGCau--GAC- -5' |
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| 22253 | 5' | -58 | NC_005045.1 | + | 25634 | 0.66 | 0.463231 |
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Target: 5'- -cACCAGgCUGCGCUCGCGgUaUACa- -3' miRNA: 3'- uaUGGUC-GGCGUGAGCGCgGcAUGac -5' |
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| 22253 | 5' | -58 | NC_005045.1 | + | 41198 | 0.66 | 0.443222 |
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Target: 5'- uGUGCCuGCUGCugUCGUugacaGCCuGUGCa- -3' miRNA: 3'- -UAUGGuCGGCGugAGCG-----CGG-CAUGac -5' |
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| 22253 | 5' | -58 | NC_005045.1 | + | 32097 | 0.7 | 0.259622 |
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Target: 5'- -cGCUGGCCGUGCUCuGCggcucgGUCGUGCUGg -3' miRNA: 3'- uaUGGUCGGCGUGAG-CG------CGGCAUGAC- -5' |
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| 22253 | 5' | -58 | NC_005045.1 | + | 5766 | 0.7 | 0.255601 |
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Target: 5'- uGUGCCAgGCCGaCGCgaagCGCGCCGacgaguacgaaaagcUGCUGc -3' miRNA: 3'- -UAUGGU-CGGC-GUGa---GCGCGGC---------------AUGAC- -5' |
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| 22253 | 5' | -58 | NC_005045.1 | + | 27565 | 0.7 | 0.246415 |
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Target: 5'- gGUACUGGUgGCAgauauCUCGUccGCCGUGCUGg -3' miRNA: 3'- -UAUGGUCGgCGU-----GAGCG--CGGCAUGAC- -5' |
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| 22253 | 5' | -58 | NC_005045.1 | + | 13088 | 1.05 | 0.000601 |
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Target: 5'- gAUACCAGCCGCACUCGCGCCGUACUGc -3' miRNA: 3'- -UAUGGUCGGCGUGAGCGCGGCAUGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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