miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22254 5' -54.6 NC_005045.1 + 26030 0.66 0.664912
Target:  5'- uGGCCgca-GCAUuGCGAuGCUGGUGg -3'
miRNA:   3'- gCCGGaacaUGUAcUGCUcCGACCAC- -5'
22254 5' -54.6 NC_005045.1 + 6904 0.66 0.642333
Target:  5'- aGGCCcUGUGCGuUGACGAccggcacagcaGGCagGGUa -3'
miRNA:   3'- gCCGGaACAUGU-ACUGCU-----------CCGa-CCAc -5'
22254 5' -54.6 NC_005045.1 + 39495 0.67 0.629892
Target:  5'- aGGUcgcgcagCUUGagcUGCAUacgcugcucgucGGCGAGGCUGGUGu -3'
miRNA:   3'- gCCG-------GAAC---AUGUA------------CUGCUCCGACCAC- -5'
22254 5' -54.6 NC_005045.1 + 34490 0.67 0.608417
Target:  5'- uGGCCcgcUGUGCA--ACGGGGCcgagcUGGUGc -3'
miRNA:   3'- gCCGGa--ACAUGUacUGCUCCG-----ACCAC- -5'
22254 5' -54.6 NC_005045.1 + 13228 1.09 0.000771
Target:  5'- uCGGCCUUGUACAUGACGAGGCUGGUGg -3'
miRNA:   3'- -GCCGGAACAUGUACUGCUCCGACCAC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.