Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22255 | 5' | -53.5 | NC_005045.1 | + | 19828 | 0.66 | 0.738919 |
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Target: 5'- uUCAaGGgCGUCGUGGACCaCuGCCUg -3' miRNA: 3'- gAGUgCCaGUAGUACUUGG-GuCGGAg -5' |
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| 22255 | 5' | -53.5 | NC_005045.1 | + | 1778 | 0.66 | 0.727973 |
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Target: 5'- cCUCACuaGGUCuGUCucuUGAAUCCccucgguggaagGGCCUCa -3' miRNA: 3'- -GAGUG--CCAG-UAGu--ACUUGGG------------UCGGAG- -5' |
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| 22255 | 5' | -53.5 | NC_005045.1 | + | 33724 | 0.67 | 0.660579 |
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Target: 5'- uUCACGGUC-UgAUGGGCCaacgGGCCa- -3' miRNA: 3'- gAGUGCCAGuAgUACUUGGg---UCGGag -5' |
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| 22255 | 5' | -53.5 | NC_005045.1 | + | 18369 | 0.67 | 0.637756 |
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Target: 5'- gUCGCGGUgAUCGuuggguaguUGAugagcaucgacaGCCCAGCC-Cg -3' miRNA: 3'- gAGUGCCAgUAGU---------ACU------------UGGGUCGGaG- -5' |
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| 22255 | 5' | -53.5 | NC_005045.1 | + | 39664 | 0.68 | 0.602367 |
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Target: 5'- -aCAUcGUCAUCugcuggaGUGGuGCCCAGCCUCc -3' miRNA: 3'- gaGUGcCAGUAG-------UACU-UGGGUCGGAG- -5' |
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| 22255 | 5' | -53.5 | NC_005045.1 | + | 17435 | 0.69 | 0.51401 |
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Target: 5'- aCUUGcCGGUCugcgUAUGcACCCAGCCUUc -3' miRNA: 3'- -GAGU-GCCAGua--GUACuUGGGUCGGAG- -5' |
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| 22255 | 5' | -53.5 | NC_005045.1 | + | 36749 | 0.72 | 0.373199 |
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Target: 5'- -cCAUGGaCAg---GGACCCAGCCUCa -3' miRNA: 3'- gaGUGCCaGUaguaCUUGGGUCGGAG- -5' |
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| 22255 | 5' | -53.5 | NC_005045.1 | + | 13448 | 1.11 | 0.000777 |
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Target: 5'- gCUCACGGUCAUCAUGAACCCAGCCUCg -3' miRNA: 3'- -GAGUGCCAGUAGUACUUGGGUCGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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