Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22256 | 3' | -56.5 | NC_005045.1 | + | 19372 | 0.66 | 0.570679 |
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Target: 5'- --gACCGGG-CAGCCuGGG--UCGAUg -3' miRNA: 3'- uaaUGGCCCuGUCGGcCCUgaAGCUG- -5' |
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| 22256 | 3' | -56.5 | NC_005045.1 | + | 14945 | 0.67 | 0.474311 |
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Target: 5'- -cUGCCugcGGGACAGCCGGuACagcaggggCGGCa -3' miRNA: 3'- uaAUGG---CCCUGUCGGCCcUGaa------GCUG- -5' |
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| 22256 | 3' | -56.5 | NC_005045.1 | + | 19492 | 0.68 | 0.453975 |
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Target: 5'- --gACCcGGAUAGuucuCCGGGcCUUCGGCg -3' miRNA: 3'- uaaUGGcCCUGUC----GGCCCuGAAGCUG- -5' |
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| 22256 | 3' | -56.5 | NC_005045.1 | + | 35412 | 0.68 | 0.41478 |
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Target: 5'- --gACCGGGGCGGacauCCGGGAgUgcccggUGACu -3' miRNA: 3'- uaaUGGCCCUGUC----GGCCCUgAa-----GCUG- -5' |
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| 22256 | 3' | -56.5 | NC_005045.1 | + | 23531 | 0.69 | 0.377734 |
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Target: 5'- --gGCCGGcGGCGG-CGGGACUUCc-- -3' miRNA: 3'- uaaUGGCC-CUGUCgGCCCUGAAGcug -5' |
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| 22256 | 3' | -56.5 | NC_005045.1 | + | 21851 | 0.7 | 0.351447 |
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Target: 5'- --cGCCGcgaggccGACAGCCGGGACacaGACa -3' miRNA: 3'- uaaUGGCc------CUGUCGGCCCUGaagCUG- -5' |
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| 22256 | 3' | -56.5 | NC_005045.1 | + | 34180 | 0.7 | 0.351447 |
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Target: 5'- -gUAUCGGGuGCAGgaccugaucgaCCGGGACcUCGACc -3' miRNA: 3'- uaAUGGCCC-UGUC-----------GGCCCUGaAGCUG- -5' |
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| 22256 | 3' | -56.5 | NC_005045.1 | + | 26825 | 0.7 | 0.318463 |
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Target: 5'- -aUACCGGGGCcuugguggAGCCGGuGGCcaUGACa -3' miRNA: 3'- uaAUGGCCCUG--------UCGGCC-CUGaaGCUG- -5' |
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| 22256 | 3' | -56.5 | NC_005045.1 | + | 28538 | 0.73 | 0.22754 |
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Target: 5'- --cGCCGaGGcucaGGCCGGGGCgUUCGGCg -3' miRNA: 3'- uaaUGGC-CCug--UCGGCCCUG-AAGCUG- -5' |
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| 22256 | 3' | -56.5 | NC_005045.1 | + | 15097 | 1.08 | 0.000571 |
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Target: 5'- gAUUACCGGGACAGCCGGGACUUCGACg -3' miRNA: 3'- -UAAUGGCCCUGUCGGCCCUGAAGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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