miRNA: 3'- cacCU-CCUaCAGCuUCCGGCAGAAGU- -5'
miRNA: 3'- cACCUCCUacaGCUUcCGGCAGAAGu -5'
miRNA: 3'- caCC-----UCCuACAGCUUCcGGCAGAAGU- -5'
miRNA: 3'- -CACCuCCUAC-AGCU-UCCGGCagaAGU- -5'
miRNA: 3'- -CACCUCCUACAGCUUCCGGCAGAAGU- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22257 | 5' | -53.8 | NC_005045.1 | + | 35584 | 0.66 | 0.729743 |
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Target: 5'- --aGAuGGG-GUCG-AGGCUGUCUUCAg -3' miRNA: 3'- cacCU-CCUaCAGCuUCCGGCAGAAGU- -5' |
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| 22257 | 5' | -53.8 | NC_005045.1 | + | 6434 | 0.68 | 0.583354 |
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Target: 5'- cUGGAGGA---CGAA-GCCGUCUUCc -3' miRNA: 3'- cACCUCCUacaGCUUcCGGCAGAAGu -5' |
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| 22257 | 5' | -53.8 | NC_005045.1 | + | 28473 | 0.7 | 0.474169 |
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Target: 5'- -cGGcgcccAGGuUGUCGAGGuCCGUCUUCAg -3' miRNA: 3'- caCC-----UCCuACAGCUUCcGGCAGAAGU- -5' |
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| 22257 | 5' | -53.8 | NC_005045.1 | + | 2446 | 0.72 | 0.376187 |
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Target: 5'- uGUGGuGGAUGaUCGAcAGGCCGg--UCAg -3' miRNA: 3'- -CACCuCCUAC-AGCU-UCCGGCagaAGU- -5' |
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| 22257 | 5' | -53.8 | NC_005045.1 | + | 15822 | 1.08 | 0.001105 |
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Target: 5'- gGUGGAGGAUGUCGAAGGCCGUCUUCAu -3' miRNA: 3'- -CACCUCCUACAGCUUCCGGCAGAAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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