Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22258 | 5' | -59.7 | NC_005045.1 | + | 30826 | 0.67 | 0.283685 |
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Target: 5'- cUUGUgCUGCUCGGCCaggACGGUUcCGAu -3' miRNA: 3'- -AACGgGACGAGCCGGg--UGUCGAaGUU- -5' |
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| 22258 | 5' | -59.7 | NC_005045.1 | + | 5304 | 0.67 | 0.283685 |
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Target: 5'- cUGCCCgcUGCUggcacaCGGCCaggGCAGCUUCc- -3' miRNA: 3'- aACGGG--ACGA------GCCGGg--UGUCGAAGuu -5' |
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| 22258 | 5' | -59.7 | NC_005045.1 | + | 35958 | 0.67 | 0.262344 |
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Target: 5'- -gGCgCUGCgcCGuGCCCugGGCUUCGc -3' miRNA: 3'- aaCGgGACGa-GC-CGGGugUCGAAGUu -5' |
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| 22258 | 5' | -59.7 | NC_005045.1 | + | 41906 | 0.68 | 0.255526 |
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Target: 5'- gUGCgaCCaGCUCaGCCCGCAGCUUgGu -3' miRNA: 3'- aACG--GGaCGAGcCGGGUGUCGAAgUu -5' |
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| 22258 | 5' | -59.7 | NC_005045.1 | + | 5641 | 0.68 | 0.255526 |
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Target: 5'- aUGCCCgGCUCuuGGCCaCGCGGCg---- -3' miRNA: 3'- aACGGGaCGAG--CCGG-GUGUCGaaguu -5' |
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| 22258 | 5' | -59.7 | NC_005045.1 | + | 10152 | 0.68 | 0.2297 |
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Target: 5'- cUGCCCUGgaCGGCCUGgAGgaUCGc -3' miRNA: 3'- aACGGGACgaGCCGGGUgUCgaAGUu -5' |
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| 22258 | 5' | -59.7 | NC_005045.1 | + | 9543 | 0.68 | 0.2297 |
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Target: 5'- -cGCCCUGUaCGGCaugaCGCAGUaUCAGg -3' miRNA: 3'- aaCGGGACGaGCCGg---GUGUCGaAGUU- -5' |
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| 22258 | 5' | -59.7 | NC_005045.1 | + | 30294 | 0.76 | 0.063447 |
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Target: 5'- -cGCCCUGCUCGGCCUccgggauaccugAUAGCaUCAc -3' miRNA: 3'- aaCGGGACGAGCCGGG------------UGUCGaAGUu -5' |
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| 22258 | 5' | -59.7 | NC_005045.1 | + | 16131 | 1.04 | 0.000401 |
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Target: 5'- uUUGCCCUGCUCGGCCCACAGCUUCAAg -3' miRNA: 3'- -AACGGGACGAGCCGGGUGUCGAAGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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