Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22259 | 3' | -59.1 | NC_005045.1 | + | 6824 | 0.7 | 0.260986 |
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Target: 5'- -gGGAgGCGugCugGCCCucgguGCCUUCGAc -3' miRNA: 3'- ggUCU-CGCugGugUGGGu----CGGGAGCU- -5' |
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| 22259 | 3' | -59.1 | NC_005045.1 | + | 32658 | 0.71 | 0.206891 |
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Target: 5'- gCAGGGCGGCgaCACugCCAGCUCUg-- -3' miRNA: 3'- gGUCUCGCUG--GUGugGGUCGGGAgcu -5' |
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| 22259 | 3' | -59.1 | NC_005045.1 | + | 27695 | 0.72 | 0.201519 |
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Target: 5'- cCCAGGGCGAUCu--CCUGGCCCUUc- -3' miRNA: 3'- -GGUCUCGCUGGuguGGGUCGGGAGcu -5' |
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| 22259 | 3' | -59.1 | NC_005045.1 | + | 10731 | 0.72 | 0.201519 |
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Target: 5'- gCCuGAGCGAggagCACAUCCAGCuCCUCa- -3' miRNA: 3'- -GGuCUCGCUg---GUGUGGGUCG-GGAGcu -5' |
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| 22259 | 3' | -59.1 | NC_005045.1 | + | 25019 | 0.72 | 0.196268 |
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Target: 5'- aCAGGGCGAUCugGCGCUCGGaCuCCUCGGc -3' miRNA: 3'- gGUCUCGCUGG--UGUGGGUC-G-GGAGCU- -5' |
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| 22259 | 3' | -59.1 | NC_005045.1 | + | 5054 | 0.77 | 0.088298 |
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Target: 5'- cCCAGGGCGAguaaggcCCGCacguaauagGCCCGGCCCUCc- -3' miRNA: 3'- -GGUCUCGCU-------GGUG---------UGGGUCGGGAGcu -5' |
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| 22259 | 3' | -59.1 | NC_005045.1 | + | 16823 | 1.1 | 0.000291 |
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Target: 5'- uCCAGAGCGACCACACCCAGCCCUCGAu -3' miRNA: 3'- -GGUCUCGCUGGUGUGGGUCGGGAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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