miRNA: 3'- aGAGCUAcaGGUCCgaGUCGUGGAag -5'
miRNA: 3'- aGAGCUaCAGGu-CCGa--GUCG------UGGAAG- -5'
miRNA: 3'- aGAgCUA---CaGGUCCGAGUCGUGgaAG- -5'
miRNA: 3'- aGAGCUA------CAGGUCCGAG-----UCGUGGaag -5'
miRNA: 3'- -AGAGCUACAGGUCCGAGUCGUGGAAG- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22259 | 5' | -54.5 | NC_005045.1 | + | 27271 | 0.66 | 0.622799 |
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Target: 5'- cCUCGAUa-CCAGGg-CGGCACCUg- -3' miRNA: 3'- aGAGCUAcaGGUCCgaGUCGUGGAag -5' |
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| 22259 | 5' | -54.5 | NC_005045.1 | + | 5914 | 0.68 | 0.521842 |
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Target: 5'- cCUCGAaGUCCuuGGCggaCAGCgggccgGCCUUCu -3' miRNA: 3'- aGAGCUaCAGGu-CCGa--GUCG------UGGAAG- -5' |
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| 22259 | 5' | -54.5 | NC_005045.1 | + | 23672 | 0.69 | 0.489494 |
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Target: 5'- cCUgGGUccgGcCCAGGCUCAGCACg-UCa -3' miRNA: 3'- aGAgCUA---CaGGUCCGAGUCGUGgaAG- -5' |
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| 22259 | 5' | -54.5 | NC_005045.1 | + | 32727 | 0.72 | 0.336111 |
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Target: 5'- cCUCGAUacccugGUCCAGGUUCgcggcAGCACCg-- -3' miRNA: 3'- aGAGCUA------CAGGUCCGAG-----UCGUGGaag -5' |
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| 22259 | 5' | -54.5 | NC_005045.1 | + | 16788 | 1.11 | 0.000542 |
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Target: 5'- gUCUCGAUGUCCAGGCUCAGCACCUUCg -3' miRNA: 3'- -AGAGCUACAGGUCCGAGUCGUGGAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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