Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2226 | 3' | -56.5 | NC_001405.1 | + | 13981 | 0.66 | 0.536156 |
Target: 5'- aGACCCcgcugacggucGUGCcuuugacgacggguGGGCGgGCGCggGCCGg -3' miRNA: 3'- gUUGGG-----------UAUG--------------CUCGCgCGCGaaCGGC- -5' |
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2226 | 3' | -56.5 | NC_001405.1 | + | 17841 | 0.66 | 0.496267 |
Target: 5'- -cGCgCAUGCGAcgguGCGaCGCGCgccGCCGc -3' miRNA: 3'- guUGgGUAUGCU----CGC-GCGCGaa-CGGC- -5' |
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2226 | 3' | -56.5 | NC_001405.1 | + | 18077 | 0.67 | 0.464936 |
Target: 5'- --uCCCAUgaACGGGCGCGaGCcgUGUCGc -3' miRNA: 3'- guuGGGUA--UGCUCGCGCgCGa-ACGGC- -5' |
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2226 | 3' | -56.5 | NC_001405.1 | + | 15168 | 0.68 | 0.415131 |
Target: 5'- ---gCCAcACGGGCggaggagaaGCGCGCUgagGCCGa -3' miRNA: 3'- guugGGUaUGCUCG---------CGCGCGAa--CGGC- -5' |
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2226 | 3' | -56.5 | NC_001405.1 | + | 16290 | 0.69 | 0.359019 |
Target: 5'- uCGGCCgGUGCGAcGUGCGCGgUUaagcaggGCCGc -3' miRNA: 3'- -GUUGGgUAUGCU-CGCGCGCgAA-------CGGC- -5' |
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2226 | 3' | -56.5 | NC_001405.1 | + | 23302 | 0.69 | 0.334266 |
Target: 5'- gAGCCC--ACGGGCuGCGCGUUgugGCUGc -3' miRNA: 3'- gUUGGGuaUGCUCG-CGCGCGAa--CGGC- -5' |
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2226 | 3' | -56.5 | NC_001405.1 | + | 12257 | 0.7 | 0.309999 |
Target: 5'- -cGCCgCcgACGuugccAGCGCGCGCgggUGCCa -3' miRNA: 3'- guUGG-GuaUGC-----UCGCGCGCGa--ACGGc -5' |
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2226 | 3' | -56.5 | NC_001405.1 | + | 3702 | 0.71 | 0.265548 |
Target: 5'- cCGGCCCAUGgGGGCaUGCGCgUUGUCa -3' miRNA: 3'- -GUUGGGUAUgCUCGcGCGCG-AACGGc -5' |
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2226 | 3' | -56.5 | NC_001405.1 | + | 5454 | 0.72 | 0.232569 |
Target: 5'- cCAGCCCcuccgcgGCGuggcccuuGGCGCGCaGCUUGCCc -3' miRNA: 3'- -GUUGGGua-----UGC--------UCGCGCG-CGAACGGc -5' |
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2226 | 3' | -56.5 | NC_001405.1 | + | 12313 | 0.72 | 0.220374 |
Target: 5'- gGACCCggcgGUGCGGGCG-GCGCUgcagaGCCa -3' miRNA: 3'- gUUGGG----UAUGCUCGCgCGCGAa----CGGc -5' |
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2226 | 3' | -56.5 | NC_001405.1 | + | 6702 | 1.07 | 0.000553 |
Target: 5'- uCAACCCAUACGAGCGCGCGCUUGCCGc -3' miRNA: 3'- -GUUGGGUAUGCUCGCGCGCGAACGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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