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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22260 | 5' | -53.2 | NC_005045.1 | + | 22904 | 0.66 | 0.775862 |
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Target: 5'- ---gCGGGacAGGGCgGGAAGGCCcGUc -3' miRNA: 3'- agaaGCCCa-UCCUGaCCUUCUGGaCG- -5' |
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| 22260 | 5' | -53.2 | NC_005045.1 | + | 32158 | 0.66 | 0.774827 |
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Target: 5'- --gUCGGGUAuguugcuGGGCUGGAugccgcagGGGCCgacGUg -3' miRNA: 3'- agaAGCCCAU-------CCUGACCU--------UCUGGa--CG- -5' |
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| 22260 | 5' | -53.2 | NC_005045.1 | + | 37115 | 0.66 | 0.774826 |
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Target: 5'- --cUCGGccaGGGACUGGccuuggaAGGAaCCUGCg -3' miRNA: 3'- agaAGCCca-UCCUGACC-------UUCU-GGACG- -5' |
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| 22260 | 5' | -53.2 | NC_005045.1 | + | 23460 | 0.66 | 0.744176 |
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Target: 5'- gCUgagCGGGUGcuugcGGAacaacgGGuuGACCUGCg -3' miRNA: 3'- aGAa--GCCCAU-----CCUga----CCuuCUGGACG- -5' |
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| 22260 | 5' | -53.2 | NC_005045.1 | + | 36274 | 0.67 | 0.711405 |
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Target: 5'- aUCUUCGGGgcGa--UGGAGGACgacuaaUGCg -3' miRNA: 3'- -AGAAGCCCauCcugACCUUCUGg-----ACG- -5' |
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| 22260 | 5' | -53.2 | NC_005045.1 | + | 9304 | 0.67 | 0.677886 |
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Target: 5'- uUCUUCGGcaGGGACaGGccacgcauGACCUGUu -3' miRNA: 3'- -AGAAGCCcaUCCUGaCCuu------CUGGACG- -5' |
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| 22260 | 5' | -53.2 | NC_005045.1 | + | 30429 | 0.68 | 0.609911 |
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Target: 5'- aUCUUCGGucGGGcCUGGAAcaugcuGACCgGCg -3' miRNA: 3'- -AGAAGCCcaUCCuGACCUU------CUGGaCG- -5' |
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| 22260 | 5' | -53.2 | NC_005045.1 | + | 20122 | 1.13 | 0.00059 |
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Target: 5'- gUCUUCGGGUAGGACUGGAAGACCUGCa -3' miRNA: 3'- -AGAAGCCCAUCCUGACCUUCUGGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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