Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22264 | 5' | -54.2 | NC_005045.1 | + | 40548 | 0.74 | 0.236887 |
Target: 5'- cCGGCCUUG-UCGcCCUGGA--AGGCAGu -3' miRNA: 3'- -GUCGGAACuAGU-GGACCUagUCCGUC- -5' |
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22264 | 5' | -54.2 | NC_005045.1 | + | 24321 | 0.69 | 0.486616 |
Target: 5'- -cGCCUgGAUCGCCUGGGcaacgUCgAGGCc- -3' miRNA: 3'- guCGGAaCUAGUGGACCU-----AG-UCCGuc -5' |
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22264 | 5' | -54.2 | NC_005045.1 | + | 31138 | 0.68 | 0.519049 |
Target: 5'- gGGCCUgggGcAUUACCUGGGcaguggcaaugcUgGGGCAGg -3' miRNA: 3'- gUCGGAa--C-UAGUGGACCU------------AgUCCGUC- -5' |
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22264 | 5' | -54.2 | NC_005045.1 | + | 41023 | 0.68 | 0.552302 |
Target: 5'- gGGCCUUGGguaUCGCCcagggGGAgaUCAagcGGCAGa -3' miRNA: 3'- gUCGGAACU---AGUGGa----CCU--AGU---CCGUC- -5' |
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22264 | 5' | -54.2 | NC_005045.1 | + | 6217 | 0.66 | 0.654742 |
Target: 5'- gCGGCUaagGA-CACCUGG--CAGGCAGc -3' miRNA: 3'- -GUCGGaa-CUaGUGGACCuaGUCCGUC- -5' |
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22264 | 5' | -54.2 | NC_005045.1 | + | 24609 | 1.09 | 0.00073 |
Target: 5'- gCAGCCUUGAUCACCUGGAUCAGGCAGg -3' miRNA: 3'- -GUCGGAACUAGUGGACCUAGUCCGUC- -5' |
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22264 | 5' | -54.2 | NC_005045.1 | + | 10350 | 0.74 | 0.250024 |
Target: 5'- uCGGCCUUGGUCAgCgGGGUCuuGCGGg -3' miRNA: 3'- -GUCGGAACUAGUgGaCCUAGucCGUC- -5' |
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22264 | 5' | -54.2 | NC_005045.1 | + | 22027 | 0.71 | 0.39579 |
Target: 5'- uCAGUagCUUGAUCACCUGcGUCAGGaccgcCAGg -3' miRNA: 3'- -GUCG--GAACUAGUGGACcUAGUCC-----GUC- -5' |
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22264 | 5' | -54.2 | NC_005045.1 | + | 31416 | 0.69 | 0.455175 |
Target: 5'- --uCCUUGAUCGCCUGGA--AGGUg- -3' miRNA: 3'- gucGGAACUAGUGGACCUagUCCGuc -5' |
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22264 | 5' | -54.2 | NC_005045.1 | + | 23307 | 0.69 | 0.508138 |
Target: 5'- gAGCCUgcugcugGAUCACC-GGGUUcGGCAu -3' miRNA: 3'- gUCGGAa------CUAGUGGaCCUAGuCCGUc -5' |
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22264 | 5' | -54.2 | NC_005045.1 | + | 30178 | 0.68 | 0.541139 |
Target: 5'- uUAGCCUcgccuuccuUGGcCGCCUGGAUCAuGCGa -3' miRNA: 3'- -GUCGGA---------ACUaGUGGACCUAGUcCGUc -5' |
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22264 | 5' | -54.2 | NC_005045.1 | + | 29480 | 0.67 | 0.574826 |
Target: 5'- aCAGCCUUGGcCAggauacuguuacCCUGGGUacggaagccaCGGGCGGc -3' miRNA: 3'- -GUCGGAACUaGU------------GGACCUA----------GUCCGUC- -5' |
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22264 | 5' | -54.2 | NC_005045.1 | + | 20319 | 0.67 | 0.608968 |
Target: 5'- -uGUCgugGAUgGCCUGGAUCGGGa-- -3' miRNA: 3'- guCGGaa-CUAgUGGACCUAGUCCguc -5' |
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22264 | 5' | -54.2 | NC_005045.1 | + | 23793 | 0.66 | 0.663877 |
Target: 5'- aCAGCCUUGGUCugCgcaucgacauaGGUCAGGaCGc -3' miRNA: 3'- -GUCGGAACUAGugGac---------CUAGUCC-GUc -5' |
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22264 | 5' | -54.2 | NC_005045.1 | + | 14125 | 0.66 | 0.666158 |
Target: 5'- gCAGCCUUGuUgGCCaGGGcgaGGGCGGc -3' miRNA: 3'- -GUCGGAACuAgUGGaCCUag-UCCGUC- -5' |
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22264 | 5' | -54.2 | NC_005045.1 | + | 35562 | 0.66 | 0.684346 |
Target: 5'- gCAGCaaCUUGGUCGCCgGGAauacguugcgaaAGGCAGu -3' miRNA: 3'- -GUCG--GAACUAGUGGaCCUag----------UCCGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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