Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22265 | 3' | -54.3 | NC_005045.1 | + | 42248 | 0.66 | 0.673878 |
Target: 5'- cGCCCUgCGGUg----GGCuACCAGCGc -3' miRNA: 3'- aUGGGAaGUCAagcuaCCG-UGGUCGC- -5' |
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22265 | 3' | -54.3 | NC_005045.1 | + | 185 | 0.66 | 0.673878 |
Target: 5'- cGCCCUgCGGUg----GGCuACCAGCGc -3' miRNA: 3'- aUGGGAaGUCAagcuaCCG-UGGUCGC- -5' |
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22265 | 3' | -54.3 | NC_005045.1 | + | 11830 | 0.67 | 0.6283 |
Target: 5'- gGCCCUgaUCGGUUucucggCGAUGGaguguGCCGGCc -3' miRNA: 3'- aUGGGA--AGUCAA------GCUACCg----UGGUCGc -5' |
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22265 | 3' | -54.3 | NC_005045.1 | + | 7164 | 0.67 | 0.616886 |
Target: 5'- --gCCUUCAGUcUGGUGGC-CCugGGCGu -3' miRNA: 3'- augGGAAGUCAaGCUACCGuGG--UCGC- -5' |
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22265 | 3' | -54.3 | NC_005045.1 | + | 2928 | 0.67 | 0.605487 |
Target: 5'- gACuCCUggcgGGUUCGugguagugGGCGCCGGCGu -3' miRNA: 3'- aUG-GGAag--UCAAGCua------CCGUGGUCGC- -5' |
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22265 | 3' | -54.3 | NC_005045.1 | + | 38387 | 0.67 | 0.594113 |
Target: 5'- -gUCCUUgAGgaacUUGGUGGCGgCAGCGa -3' miRNA: 3'- auGGGAAgUCa---AGCUACCGUgGUCGC- -5' |
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22265 | 3' | -54.3 | NC_005045.1 | + | 8661 | 0.68 | 0.537945 |
Target: 5'- gGCCCUUCAuGUaCGccGGCagcACCAGCc -3' miRNA: 3'- aUGGGAAGU-CAaGCuaCCG---UGGUCGc -5' |
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22265 | 3' | -54.3 | NC_005045.1 | + | 37285 | 0.68 | 0.537945 |
Target: 5'- aUACCCUUCAucGUgaucUUGAcuucacUGGaCGCCGGCGg -3' miRNA: 3'- -AUGGGAAGU--CA----AGCU------ACC-GUGGUCGC- -5' |
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22265 | 3' | -54.3 | NC_005045.1 | + | 30825 | 0.68 | 0.526913 |
Target: 5'- --gCCUUCgAGUaCGAccGCACCAGCGg -3' miRNA: 3'- augGGAAG-UCAaGCUacCGUGGUCGC- -5' |
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22265 | 3' | -54.3 | NC_005045.1 | + | 10250 | 0.68 | 0.526913 |
Target: 5'- aUGCCCUUCAGUgaggCGAccgGGCguaugGCCcGCa -3' miRNA: 3'- -AUGGGAAGUCAa---GCUa--CCG-----UGGuCGc -5' |
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22265 | 3' | -54.3 | NC_005045.1 | + | 34665 | 0.68 | 0.525815 |
Target: 5'- aGCCCUggaagcgUCAGUUCGAuaccuuccUGGCcaGCCAGg- -3' miRNA: 3'- aUGGGA-------AGUCAAGCU--------ACCG--UGGUCgc -5' |
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22265 | 3' | -54.3 | NC_005045.1 | + | 15109 | 0.68 | 0.515968 |
Target: 5'- cUACCCgagCAGUUCGAccgcagcaUGGCA--GGCGg -3' miRNA: 3'- -AUGGGaa-GUCAAGCU--------ACCGUggUCGC- -5' |
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22265 | 3' | -54.3 | NC_005045.1 | + | 41835 | 0.69 | 0.505115 |
Target: 5'- -----aUCAGguucUCGAUGGUGCCGGCGa -3' miRNA: 3'- augggaAGUCa---AGCUACCGUGGUCGC- -5' |
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22265 | 3' | -54.3 | NC_005045.1 | + | 25935 | 0.69 | 0.483713 |
Target: 5'- gGCCCggCAGgcgcUCGAagGGCACCuGCu -3' miRNA: 3'- aUGGGaaGUCa---AGCUa-CCGUGGuCGc -5' |
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22265 | 3' | -54.3 | NC_005045.1 | + | 37640 | 0.69 | 0.452459 |
Target: 5'- gUGCCCUUcCAGggugCGcgGG-ACCGGCGg -3' miRNA: 3'- -AUGGGAA-GUCaa--GCuaCCgUGGUCGC- -5' |
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22265 | 3' | -54.3 | NC_005045.1 | + | 20978 | 0.82 | 0.072525 |
Target: 5'- gGCCCaUCAGUUCGGauggGGCGCCGGCc -3' miRNA: 3'- aUGGGaAGUCAAGCUa---CCGUGGUCGc -5' |
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22265 | 3' | -54.3 | NC_005045.1 | + | 25179 | 1.08 | 0.000862 |
Target: 5'- aUACCCUUCAGUUCGAUGGCACCAGCGg -3' miRNA: 3'- -AUGGGAAGUCAAGCUACCGUGGUCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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