miRNA: 3'- -GAC-GAGCGACUcgACCGGGCCGUCCG- -5'
miRNA: 3'- gaCGAGCGACUCGACCG------------GGCCGUCCg -5'
miRNA: 3'- -GACGAGCGACUCGACCGGGCCGUCCG- -5'
Results 21 - 23 of 23 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22267 | 3' | -62.3 | NC_005045.1 | + | 33927 | 0.81 | 0.020075 |
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Target: 5'- aCUGcCUCGCUGA--UGGCUCGGCGGGCc -3' miRNA: 3'- -GAC-GAGCGACUcgACCGGGCCGUCCG- -5' |
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| 22267 | 3' | -62.3 | NC_005045.1 | + | 34494 | 0.84 | 0.011525 |
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Target: 5'- -aGCUUGCUGAGCUGGCcacgauggcccaCCGGCAGGg -3' miRNA: 3'- gaCGAGCGACUCGACCG------------GGCCGUCCg -5' |
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| 22267 | 3' | -62.3 | NC_005045.1 | + | 25950 | 1.11 | 0.00009 |
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Target: 5'- uCUGCUCGCUGAGCUGGCCCGGCAGGCg -3' miRNA: 3'- -GACGAGCGACUCGACCGGGCCGUCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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