Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22268 | 5' | -53.7 | NC_005045.1 | + | 39812 | 0.66 | 0.709451 |
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Target: 5'- gCUggUGCaGU-UGGUCAGCUCUuccGCa -3' miRNA: 3'- -GGuuACGaCGaACCAGUCGAGGu--CGa -5' |
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| 22268 | 5' | -53.7 | NC_005045.1 | + | 36433 | 0.66 | 0.70495 |
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Target: 5'- uCCGAggaaGaCUGCggguaggugauggUGGUCAGCaCCGGCUu -3' miRNA: 3'- -GGUUa---C-GACGa------------ACCAGUCGaGGUCGA- -5' |
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| 22268 | 5' | -53.7 | NC_005045.1 | + | 39372 | 0.67 | 0.606498 |
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Target: 5'- ----cGCUGCg-GGUCAGCcgcCCAGCc -3' miRNA: 3'- gguuaCGACGaaCCAGUCGa--GGUCGa -5' |
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| 22268 | 5' | -53.7 | NC_005045.1 | + | 18855 | 0.68 | 0.538394 |
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Target: 5'- ---cUGgUGCUUGGUgCGGCguaCCAGCa -3' miRNA: 3'- gguuACgACGAACCA-GUCGa--GGUCGa -5' |
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| 22268 | 5' | -53.7 | NC_005045.1 | + | 32117 | 0.7 | 0.462594 |
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Target: 5'- gCCAggGCUGCcgGGUCcAGCauaCCAGCc -3' miRNA: 3'- -GGUuaCGACGaaCCAG-UCGa--GGUCGa -5' |
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| 22268 | 5' | -53.7 | NC_005045.1 | + | 27814 | 1.09 | 0.000792 |
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Target: 5'- cCCAAUGCUGCUUGGUCAGCUCCAGCUc -3' miRNA: 3'- -GGUUACGACGAACCAGUCGAGGUCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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