Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22269 | 5' | -48.5 | NC_005045.1 | + | 32525 | 0.66 | 0.957959 |
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Target: 5'- uCACGUCGcGGGAugGCCg--GUAUcUCg -3' miRNA: 3'- cGUGCAGC-UCCUugUGGaagCAUA-AG- -5' |
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| 22269 | 5' | -48.5 | NC_005045.1 | + | 24336 | 0.66 | 0.948908 |
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Target: 5'- gGCaACGUCGAGGccaAGgGCCgUCGUGc-- -3' miRNA: 3'- -CG-UGCAGCUCC---UUgUGGaAGCAUaag -5' |
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| 22269 | 5' | -48.5 | NC_005045.1 | + | 12645 | 0.67 | 0.943932 |
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Target: 5'- cCACGUacuGGAGCAuccCCUUCGUGUc- -3' miRNA: 3'- cGUGCAgcuCCUUGU---GGAAGCAUAag -5' |
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| 22269 | 5' | -48.5 | NC_005045.1 | + | 24911 | 0.67 | 0.927155 |
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Target: 5'- cGC-CGUUGAGGAugGCCacgCGggacUUCa -3' miRNA: 3'- -CGuGCAGCUCCUugUGGaa-GCau--AAG- -5' |
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| 22269 | 5' | -48.5 | NC_005045.1 | + | 3073 | 0.68 | 0.885258 |
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Target: 5'- aGCACGUCGAa----GCCUUCGgagUCg -3' miRNA: 3'- -CGUGCAGCUccuugUGGAAGCauaAG- -5' |
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| 22269 | 5' | -48.5 | NC_005045.1 | + | 22365 | 0.72 | 0.716916 |
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Target: 5'- cCACGUCGAGGAcuACAUCggCGacUUCg -3' miRNA: 3'- cGUGCAGCUCCU--UGUGGaaGCauAAG- -5' |
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| 22269 | 5' | -48.5 | NC_005045.1 | + | 15039 | 0.74 | 0.612617 |
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Target: 5'- gGCACGUCaGGGAGCACgCUUUGggccUCg -3' miRNA: 3'- -CGUGCAGcUCCUUGUG-GAAGCaua-AG- -5' |
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| 22269 | 5' | -48.5 | NC_005045.1 | + | 27932 | 1.14 | 0.002002 |
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Target: 5'- gGCACGUCGAGGAACACCUUCGUAUUCg -3' miRNA: 3'- -CGUGCAGCUCCUUGUGGAAGCAUAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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