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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22271 | 3' | -51.6 | NC_005045.1 | + | 11660 | 0.68 | 0.744525 |
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Target: 5'- aGCUCGucgaguGCcGGGUacagCCACGUCUGAUg- -3' miRNA: 3'- -CGAGC------CGcUCCAa---GGUGUAGACUAac -5' |
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| 22271 | 3' | -51.6 | NC_005045.1 | + | 28612 | 0.69 | 0.665932 |
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Target: 5'- aGgUCGGCGAGGc-CCugAUCcaGAUUGa -3' miRNA: 3'- -CgAGCCGCUCCaaGGugUAGa-CUAAC- -5' |
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| 22271 | 3' | -51.6 | NC_005045.1 | + | 16542 | 0.72 | 0.493169 |
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Target: 5'- cGCuUCGGUGAGGUgagccggcagugcuUCCGCAaCUGGUg- -3' miRNA: 3'- -CG-AGCCGCUCCA--------------AGGUGUaGACUAac -5' |
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| 22271 | 3' | -51.6 | NC_005045.1 | + | 22394 | 0.73 | 0.443848 |
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Target: 5'- uGCUCgccaggGGCGAGGUUCCAgGUCggcacGAUg- -3' miRNA: 3'- -CGAG------CCGCUCCAAGGUgUAGa----CUAac -5' |
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| 22271 | 3' | -51.6 | NC_005045.1 | + | 28719 | 1.1 | 0.001405 |
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Target: 5'- uGCUCGGCGAGGUUCCACAUCUGAUUGu -3' miRNA: 3'- -CGAGCCGCUCCAAGGUGUAGACUAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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