Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22274 | 3' | -52.2 | NC_005045.1 | + | 24384 | 0.66 | 0.807216 |
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Target: 5'- aGCCga-GUCGUGAACGAcaaGUGGaaCCUGa -3' miRNA: 3'- -CGGaacCAGUACUUGCUc--UACC--GGAC- -5' |
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| 22274 | 3' | -52.2 | NC_005045.1 | + | 9050 | 0.66 | 0.786988 |
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Target: 5'- gGCCgaGGUCc---GCGAGAUGGCg-- -3' miRNA: 3'- -CGGaaCCAGuacuUGCUCUACCGgac -5' |
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| 22274 | 3' | -52.2 | NC_005045.1 | + | 13226 | 0.67 | 0.733565 |
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Target: 5'- gGCCUUGuaCAUG-ACGAGGcuggUGGCCUc -3' miRNA: 3'- -CGGAACcaGUACuUGCUCU----ACCGGAc -5' |
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| 22274 | 3' | -52.2 | NC_005045.1 | + | 34128 | 0.67 | 0.722495 |
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Target: 5'- aUCUUGGcCAUGAugcuguCGAacuUGGCCUGg -3' miRNA: 3'- cGGAACCaGUACUu-----GCUcu-ACCGGAC- -5' |
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| 22274 | 3' | -52.2 | NC_005045.1 | + | 26818 | 0.68 | 0.700073 |
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Target: 5'- gGCCUUGGUg--GAGCc-GGUGGCCa- -3' miRNA: 3'- -CGGAACCAguaCUUGcuCUACCGGac -5' |
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| 22274 | 3' | -52.2 | NC_005045.1 | + | 32539 | 0.69 | 0.597026 |
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Target: 5'- cGCCgaaGGcCGUGucacgucGCGGGAUGGCCg- -3' miRNA: 3'- -CGGaa-CCaGUACu------UGCUCUACCGGac -5' |
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| 22274 | 3' | -52.2 | NC_005045.1 | + | 27331 | 0.7 | 0.574204 |
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Target: 5'- uGCCcUGGguuUCAUGGGCcugcauGAGAUGGCCc- -3' miRNA: 3'- -CGGaACC---AGUACUUG------CUCUACCGGac -5' |
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| 22274 | 3' | -52.2 | NC_005045.1 | + | 10348 | 0.71 | 0.485658 |
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Target: 5'- gGCCUUGGUCA---GCGGGGUcuugcgGGCCa- -3' miRNA: 3'- -CGGAACCAGUacuUGCUCUA------CCGGac -5' |
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| 22274 | 3' | -52.2 | NC_005045.1 | + | 29906 | 1.11 | 0.000996 |
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Target: 5'- gGCCUUGGUCAUGAACGAGAUGGCCUGa -3' miRNA: 3'- -CGGAACCAGUACUUGCUCUACCGGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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