Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22274 | 5' | -60.9 | NC_005045.1 | + | 41539 | 0.66 | 0.358268 |
Target: 5'- cGGUGGCCUUggccuugaaucggCCUCGuCCAUGUCguguagggcgauGCGGu -3' miRNA: 3'- -UCGCCGGGA-------------GGAGCuGGUACGG------------UGCC- -5' |
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22274 | 5' | -60.9 | NC_005045.1 | + | 7208 | 0.66 | 0.342701 |
Target: 5'- cGGCGGCCCgCCUaCGGCgcccgCAUGaCCugGc -3' miRNA: 3'- -UCGCCGGGaGGA-GCUG-----GUAC-GGugCc -5' |
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22274 | 5' | -60.9 | NC_005045.1 | + | 10793 | 0.66 | 0.376042 |
Target: 5'- uGGauGUgCUCCUCGcucagGCCGguggcgGCCACGGu -3' miRNA: 3'- -UCgcCGgGAGGAGC-----UGGUa-----CGGUGCC- -5' |
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22274 | 5' | -60.9 | NC_005045.1 | + | 30177 | 0.66 | 0.384713 |
Target: 5'- uAGCcucGCCUUCCUUGGCC--GCCugGa -3' miRNA: 3'- -UCGc--CGGGAGGAGCUGGuaCGGugCc -5' |
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22274 | 5' | -60.9 | NC_005045.1 | + | 12996 | 0.66 | 0.384713 |
Target: 5'- uGgGGUCCUUCUUGAUg--GCCACGa -3' miRNA: 3'- uCgCCGGGAGGAGCUGguaCGGUGCc -5' |
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22274 | 5' | -60.9 | NC_005045.1 | + | 6258 | 0.66 | 0.342701 |
Target: 5'- cGCcuucGCCCgcucacgagCCUCGGCCucagccuucGCCACGGa -3' miRNA: 3'- uCGc---CGGGa--------GGAGCUGGua-------CGGUGCC- -5' |
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22274 | 5' | -60.9 | NC_005045.1 | + | 3318 | 0.66 | 0.384713 |
Target: 5'- cGGCuuGGUCacagCCUCGACgAUuCCACGGg -3' miRNA: 3'- -UCG--CCGGga--GGAGCUGgUAcGGUGCC- -5' |
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22274 | 5' | -60.9 | NC_005045.1 | + | 11475 | 0.67 | 0.304101 |
Target: 5'- cAGCGGUuguucgggucuUCggCUUCGGCCcgGCUGCGGa -3' miRNA: 3'- -UCGCCG-----------GGa-GGAGCUGGuaCGGUGCC- -5' |
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22274 | 5' | -60.9 | NC_005045.1 | + | 17903 | 0.67 | 0.311546 |
Target: 5'- cGCGGaccagCCUCCUCGGUCAggGCCACc- -3' miRNA: 3'- uCGCCg----GGAGGAGCUGGUa-CGGUGcc -5' |
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22274 | 5' | -60.9 | NC_005045.1 | + | 26873 | 0.67 | 0.319128 |
Target: 5'- aGGCGGCCCg-CUCuGCCcaGCgGCGGu -3' miRNA: 3'- -UCGCCGGGagGAGcUGGuaCGgUGCC- -5' |
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22274 | 5' | -60.9 | NC_005045.1 | + | 33893 | 0.67 | 0.337888 |
Target: 5'- gGGCGGCCUcauccaucugagccaUCUUgGACCAguuCCACGa -3' miRNA: 3'- -UCGCCGGG---------------AGGAgCUGGUac-GGUGCc -5' |
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22274 | 5' | -60.9 | NC_005045.1 | + | 2368 | 0.68 | 0.289623 |
Target: 5'- cGCGGUUgaUCUCuuCUGUGCCACGGa -3' miRNA: 3'- uCGCCGGgaGGAGcuGGUACGGUGCC- -5' |
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22274 | 5' | -60.9 | NC_005045.1 | + | 34180 | 0.68 | 0.282589 |
Target: 5'- cAGuCGGCCCUUgccguaCUUGACgGUGCCcugguCGGa -3' miRNA: 3'- -UC-GCCGGGAG------GAGCUGgUACGGu----GCC- -5' |
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22274 | 5' | -60.9 | NC_005045.1 | + | 7121 | 0.68 | 0.275692 |
Target: 5'- -cCGGCUCUCCUgcUGGCCAguucGCCucGCGGu -3' miRNA: 3'- ucGCCGGGAGGA--GCUGGUa---CGG--UGCC- -5' |
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22274 | 5' | -60.9 | NC_005045.1 | + | 41809 | 0.68 | 0.262301 |
Target: 5'- aAGCGGCCCUggguUCUggcuacaacgaGACCAaGCUGCGGg -3' miRNA: 3'- -UCGCCGGGA----GGAg----------CUGGUaCGGUGCC- -5' |
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22274 | 5' | -60.9 | NC_005045.1 | + | 40334 | 0.69 | 0.237113 |
Target: 5'- gGGCGGCCCuugcgacgcUCC-CGGcCCAUGCCGa-- -3' miRNA: 3'- -UCGCCGGG---------AGGaGCU-GGUACGGUgcc -5' |
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22274 | 5' | -60.9 | NC_005045.1 | + | 13581 | 0.69 | 0.219576 |
Target: 5'- cGCaGCCCUUgUUGACCucgGCgGCGGu -3' miRNA: 3'- uCGcCGGGAGgAGCUGGua-CGgUGCC- -5' |
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22274 | 5' | -60.9 | NC_005045.1 | + | 33965 | 0.69 | 0.225296 |
Target: 5'- cGGCGucaGCCCggUCCa-GGCCAgggaUGCCACGGa -3' miRNA: 3'- -UCGC---CGGG--AGGagCUGGU----ACGGUGCC- -5' |
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22274 | 5' | -60.9 | NC_005045.1 | + | 13031 | 0.71 | 0.164512 |
Target: 5'- -uCGaGCCUgcaUCaugCGACCGUGCCACGGg -3' miRNA: 3'- ucGC-CGGG---AGga-GCUGGUACGGUGCC- -5' |
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22274 | 5' | -60.9 | NC_005045.1 | + | 24403 | 0.72 | 0.143838 |
Target: 5'- cGaCGGCCCUuggCCUCGACguUGCCcaggcgauccagGCGGa -3' miRNA: 3'- uC-GCCGGGA---GGAGCUGguACGG------------UGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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