Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22278 | 3' | -63.3 | NC_005045.1 | + | 14398 | 0.66 | 0.281075 |
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Target: 5'- aGGCcGGGCUugaucGAGccGUCGGCCUCGu -3' miRNA: 3'- cCCGaCCCGGuc---CUC--CAGCCGGAGUg -5' |
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| 22278 | 3' | -63.3 | NC_005045.1 | + | 38006 | 0.66 | 0.274248 |
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Target: 5'- aGGGCagcgccUGGGCgCAGGuucuGGUCuGCUaCACa -3' miRNA: 3'- -CCCG------ACCCG-GUCCu---CCAGcCGGaGUG- -5' |
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| 22278 | 3' | -63.3 | NC_005045.1 | + | 40260 | 0.7 | 0.128916 |
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Target: 5'- cGGCaUGGGCCGGGAGcGUCgcaagGGCCgccCAa -3' miRNA: 3'- cCCG-ACCCGGUCCUC-CAG-----CCGGa--GUg -5' |
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| 22278 | 3' | -63.3 | NC_005045.1 | + | 33909 | 0.74 | 0.06457 |
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Target: 5'- cGGC-GGGCCAGcccaGGGG-CGGCCUCAUc -3' miRNA: 3'- cCCGaCCCGGUC----CUCCaGCCGGAGUG- -5' |
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| 22278 | 3' | -63.3 | NC_005045.1 | + | 16759 | 0.74 | 0.064389 |
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Target: 5'- aGGGCUGGGUguGGucgcucuggaagcAGaacGUCGGCCUCAa -3' miRNA: 3'- -CCCGACCCGguCC-------------UC---CAGCCGGAGUg -5' |
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| 22278 | 3' | -63.3 | NC_005045.1 | + | 33414 | 1.1 | 8.9e-05 |
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Target: 5'- cGGGCUGGGCCAGGAGGUCGGCCUCACu -3' miRNA: 3'- -CCCGACCCGGUCCUCCAGCCGGAGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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