Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22279 | 3' | -56.1 | NC_005045.1 | + | 32750 | 0.67 | 0.545771 |
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Target: 5'- uCCCGCuGUG-CCUgGCUUGaggaaggcaGAGCUGGc -3' miRNA: 3'- -GGGCG-CACuGGA-CGAGCaa-------CUCGACC- -5' |
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| 22279 | 3' | -56.1 | NC_005045.1 | + | 24846 | 0.68 | 0.524429 |
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Target: 5'- uCCCGCGUGGCCauccucaacgGCgUCaagGuGCUGGa -3' miRNA: 3'- -GGGCGCACUGGa---------CG-AGcaaCuCGACC- -5' |
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| 22279 | 3' | -56.1 | NC_005045.1 | + | 20340 | 0.69 | 0.442773 |
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Target: 5'- gCCaUGUacaGUGGCCccugcgUGCUCGUaGAGCUGGc -3' miRNA: 3'- -GG-GCG---CACUGG------ACGAGCAaCUCGACC- -5' |
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| 22279 | 3' | -56.1 | NC_005045.1 | + | 38208 | 0.73 | 0.26165 |
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Target: 5'- aCCGCGagugcugagcggcugGGCCUGCUacaUGAGCUGGu -3' miRNA: 3'- gGGCGCa--------------CUGGACGAgcaACUCGACC- -5' |
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| 22279 | 3' | -56.1 | NC_005045.1 | + | 34481 | 0.73 | 0.254351 |
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Target: 5'- uCCgGCGUGuAUCaGCUUGcUGAGCUGGc -3' miRNA: 3'- -GGgCGCAC-UGGaCGAGCaACUCGACC- -5' |
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| 22279 | 3' | -56.1 | NC_005045.1 | + | 8998 | 0.75 | 0.200771 |
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Target: 5'- aUCGCGUcaauGACCUGCUCGgccUGGGCcagGGa -3' miRNA: 3'- gGGCGCA----CUGGACGAGCa--ACUCGa--CC- -5' |
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| 22279 | 3' | -56.1 | NC_005045.1 | + | 25960 | 0.75 | 0.185219 |
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Target: 5'- aCCaUGuUGAUCUGCUCGcUGAGCUGGc -3' miRNA: 3'- gGGcGC-ACUGGACGAGCaACUCGACC- -5' |
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| 22279 | 3' | -56.1 | NC_005045.1 | + | 8452 | 0.75 | 0.180276 |
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Target: 5'- cCCUGCcUGAaaaauCCaGUUCGUUGAGCUGGg -3' miRNA: 3'- -GGGCGcACU-----GGaCGAGCAACUCGACC- -5' |
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| 22279 | 3' | -56.1 | NC_005045.1 | + | 33663 | 1.11 | 0.000467 |
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Target: 5'- uCCCGCGUGACCUGCUCGUUGAGCUGGu -3' miRNA: 3'- -GGGCGCACUGGACGAGCAACUCGACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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