Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2228 | 5' | -58.3 | NC_001405.1 | + | 28082 | 0.66 | 0.457371 |
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Target: 5'- -aGCGGGAGCggggucccugugUUCuGACCGUGGuuuGCAa -3' miRNA: 3'- ggCGCCCUCG------------AGGuCUGGUACC---UGUc -5' |
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| 2228 | 5' | -58.3 | NC_001405.1 | + | 29301 | 0.66 | 0.44745 |
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Target: 5'- aUGUGGGAuauGCUCCAGGC---GGGCAa -3' miRNA: 3'- gGCGCCCU---CGAGGUCUGguaCCUGUc -5' |
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| 2228 | 5' | -58.3 | NC_001405.1 | + | 17148 | 0.67 | 0.390573 |
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Target: 5'- aCCGgGacuGGGCgUgCAGACCGUGGACGu -3' miRNA: 3'- -GGCgCc--CUCG-AgGUCUGGUACCUGUc -5' |
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| 2228 | 5' | -58.3 | NC_001405.1 | + | 18316 | 0.68 | 0.330476 |
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Target: 5'- cCCGUaGaGGAGcCUCCAccGGCCGUGGAgaCAGu -3' miRNA: 3'- -GGCG-C-CCUC-GAGGU--CUGGUACCU--GUC- -5' |
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| 2228 | 5' | -58.3 | NC_001405.1 | + | 8599 | 0.7 | 0.247762 |
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Target: 5'- -aGgGGGGGCUCgGGACCcgccgggagaggGGGCAGg -3' miRNA: 3'- ggCgCCCUCGAGgUCUGGua----------CCUGUC- -5' |
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| 2228 | 5' | -58.3 | NC_001405.1 | + | 8403 | 1.09 | 0.000302 |
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Target: 5'- gCCGCGGGAGCUCCAGACCAUGGACAGc -3' miRNA: 3'- -GGCGCCCUCGAGGUCUGGUACCUGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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