Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22281 | 3' | -56.7 | NC_005045.1 | + | 35425 | 0.66 | 0.580962 |
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Target: 5'- --cCGCcaGCCCGaaGAC-CGGGGCGGAc -3' miRNA: 3'- guaGCG--CGGGUacUUGaGCCUCGCCU- -5' |
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| 22281 | 3' | -56.7 | NC_005045.1 | + | 23853 | 0.66 | 0.54819 |
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Target: 5'- gGUCG-GCUgAUGGACUCGG-GCGa- -3' miRNA: 3'- gUAGCgCGGgUACUUGAGCCuCGCcu -5' |
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| 22281 | 3' | -56.7 | NC_005045.1 | + | 34404 | 0.66 | 0.53739 |
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Target: 5'- --aCGUGCCgCGUGAgcaccaGCUCGGccccguugcacAGCGGGc -3' miRNA: 3'- guaGCGCGG-GUACU------UGAGCC-----------UCGCCU- -5' |
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| 22281 | 3' | -56.7 | NC_005045.1 | + | 27885 | 0.67 | 0.526665 |
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Target: 5'- --aCGUGCCCGaGGccguGgUCGGGGCGGu -3' miRNA: 3'- guaGCGCGGGUaCU----UgAGCCUCGCCu -5' |
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| 22281 | 3' | -56.7 | NC_005045.1 | + | 13586 | 0.67 | 0.474381 |
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Target: 5'- uGUCGCgcaGCCCuuguUGAcCUCGGcGGCGGu -3' miRNA: 3'- gUAGCG---CGGGu---ACUuGAGCC-UCGCCu -5' |
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| 22281 | 3' | -56.7 | NC_005045.1 | + | 24356 | 0.69 | 0.387483 |
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Target: 5'- cCGUCGUGCCgGUGAagaGCU-GGAGCGc- -3' miRNA: 3'- -GUAGCGCGGgUACU---UGAgCCUCGCcu -5' |
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| 22281 | 3' | -56.7 | NC_005045.1 | + | 26292 | 0.69 | 0.36964 |
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Target: 5'- gGUCuaCGCCCuUGAugUCGGuGCGGu -3' miRNA: 3'- gUAGc-GCGGGuACUugAGCCuCGCCu -5' |
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| 22281 | 3' | -56.7 | NC_005045.1 | + | 20359 | 0.7 | 0.360931 |
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Target: 5'- --gCGUGCUCGUaGAgcuggcgaaacaGCUCGGGGUGGAa -3' miRNA: 3'- guaGCGCGGGUA-CU------------UGAGCCUCGCCU- -5' |
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| 22281 | 3' | -56.7 | NC_005045.1 | + | 33252 | 0.71 | 0.311731 |
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Target: 5'- gCAUgGCGUCCAUGAucguCUCGGAcgccgccaggacGCGGc -3' miRNA: 3'- -GUAgCGCGGGUACUu---GAGCCU------------CGCCu -5' |
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| 22281 | 3' | -56.7 | NC_005045.1 | + | 34043 | 1.08 | 0.000585 |
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Target: 5'- cCAUCGCGCCCAUGAACUCGGAGCGGAu -3' miRNA: 3'- -GUAGCGCGGGUACUUGAGCCUCGCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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