Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22282 | 5' | -58.6 | NC_005045.1 | + | 34251 | 1.1 | 0.000363 |
Target: 5'- uCCCGGUCGAUCAGGUCCUGCACCCGAu -3' miRNA: 3'- -GGGCCAGCUAGUCCAGGACGUGGGCU- -5' |
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22282 | 5' | -58.6 | NC_005045.1 | + | 6592 | 0.73 | 0.173981 |
Target: 5'- gCCGaaGUCaAUCAGGUCCUGCGCCa-- -3' miRNA: 3'- gGGC--CAGcUAGUCCAGGACGUGGgcu -5' |
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22282 | 5' | -58.6 | NC_005045.1 | + | 15054 | 0.71 | 0.257068 |
Target: 5'- uCCCGG-CGGUgccgCAGGUCUaaUGCGCCUGc -3' miRNA: 3'- -GGGCCaGCUA----GUCCAGG--ACGUGGGCu -5' |
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22282 | 5' | -58.6 | NC_005045.1 | + | 20127 | 0.67 | 0.452277 |
Target: 5'- cCUCGGUCu-UCGGGUaggacuggaagaCCUGCAUgCCGAg -3' miRNA: 3'- -GGGCCAGcuAGUCCA------------GGACGUG-GGCU- -5' |
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22282 | 5' | -58.6 | NC_005045.1 | + | 24684 | 0.67 | 0.462032 |
Target: 5'- --aGGcCGGUCAGGUCCaGUuucuccagcacGCCCGGc -3' miRNA: 3'- gggCCaGCUAGUCCAGGaCG-----------UGGGCU- -5' |
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22282 | 5' | -58.6 | NC_005045.1 | + | 4345 | 0.66 | 0.502107 |
Target: 5'- uUCCGGUgagaUGuAUCGGGUagUGCAgCCCGAg -3' miRNA: 3'- -GGGCCA----GC-UAGUCCAggACGU-GGGCU- -5' |
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22282 | 5' | -58.6 | NC_005045.1 | + | 31670 | 0.66 | 0.512365 |
Target: 5'- aCCGcGUCGAguuGGcCCUGUACuuGGg -3' miRNA: 3'- gGGC-CAGCUaguCCaGGACGUGggCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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