Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22284 | 3' | -54.5 | NC_005045.1 | + | 15570 | 0.66 | 0.657365 |
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Target: 5'- gGAUCGgaugaugGCCUUauccuucauggcguaGGCGUCCAUCAUCa -3' miRNA: 3'- aCUAGCga-----CGGAA---------------CCGUGGGUAGUAG- -5' |
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| 22284 | 3' | -54.5 | NC_005045.1 | + | 30361 | 0.66 | 0.618427 |
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Target: 5'- cUGAacUCGCgcaggGCCUgggccggGGCaauGCCCAUCuUCa -3' miRNA: 3'- -ACU--AGCGa----CGGAa------CCG---UGGGUAGuAG- -5' |
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| 22284 | 3' | -54.5 | NC_005045.1 | + | 7714 | 0.68 | 0.543613 |
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Target: 5'- cGAU-GCUGCCUUucgcuguacagcagcGGCAgCCCuggcGUCAUCa -3' miRNA: 3'- aCUAgCGACGGAA---------------CCGU-GGG----UAGUAG- -5' |
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| 22284 | 3' | -54.5 | NC_005045.1 | + | 3325 | 0.68 | 0.517083 |
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Target: 5'- cUGAUCGaaGCCgaGGCACgCAacaugcgcuUCAUCa -3' miRNA: 3'- -ACUAGCgaCGGaaCCGUGgGU---------AGUAG- -5' |
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| 22284 | 3' | -54.5 | NC_005045.1 | + | 29050 | 0.68 | 0.495379 |
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Target: 5'- cGAUCuGCUGaCC--GGCACCCAggaugcCGUCg -3' miRNA: 3'- aCUAG-CGAC-GGaaCCGUGGGUa-----GUAG- -5' |
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| 22284 | 3' | -54.5 | NC_005045.1 | + | 24873 | 0.7 | 0.413209 |
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Target: 5'- uUGGUCGCaccgguUGCC-UGGUACUCGacguUCAUCa -3' miRNA: 3'- -ACUAGCG------ACGGaACCGUGGGU----AGUAG- -5' |
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| 22284 | 3' | -54.5 | NC_005045.1 | + | 38059 | 0.71 | 0.357387 |
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Target: 5'- aGG-CGCUGCCcucUGGcCGCCgAUCGUCu -3' miRNA: 3'- aCUaGCGACGGa--ACC-GUGGgUAGUAG- -5' |
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| 22284 | 3' | -54.5 | NC_005045.1 | + | 35279 | 1.11 | 0.000529 |
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Target: 5'- uUGAUCGCUGCCUUGGCACCCAUCAUCa -3' miRNA: 3'- -ACUAGCGACGGAACCGUGGGUAGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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