Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22285 | 5' | -57 | NC_005045.1 | + | 66 | 0.66 | 0.487499 |
Target: 5'- -cUGGGaGCUUCCggcuucGCCGGGUGGCg -3' miRNA: 3'- caGCUCaCGAAGGaca---CGGUCCGCUG- -5' |
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22285 | 5' | -57 | NC_005045.1 | + | 42129 | 0.66 | 0.487499 |
Target: 5'- -cUGGGaGCUUCCggcuucGCCGGGUGGCg -3' miRNA: 3'- caGCUCaCGAAGGaca---CGGUCCGCUG- -5' |
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22285 | 5' | -57 | NC_005045.1 | + | 31286 | 0.67 | 0.477102 |
Target: 5'- gGUCGAGUGCgcggUCgGUGUCcucGGCGAa -3' miRNA: 3'- -CAGCUCACGaa--GGaCACGGu--CCGCUg -5' |
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22285 | 5' | -57 | NC_005045.1 | + | 15664 | 0.7 | 0.304526 |
Target: 5'- gGUCGgaAGUuCUUCCUGgcgcagugGCUGGGCGGCg -3' miRNA: 3'- -CAGC--UCAcGAAGGACa-------CGGUCCGCUG- -5' |
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22285 | 5' | -57 | NC_005045.1 | + | 5749 | 0.72 | 0.210954 |
Target: 5'- -gCGGGUaGCggccagCUUGUGCCAGGcCGACg -3' miRNA: 3'- caGCUCA-CGaa----GGACACGGUCC-GCUG- -5' |
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22285 | 5' | -57 | NC_005045.1 | + | 12948 | 0.73 | 0.199811 |
Target: 5'- aGUUGuuGUGCUUCCc-UGCCAGGCGGu -3' miRNA: 3'- -CAGCu-CACGAAGGacACGGUCCGCUg -5' |
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22285 | 5' | -57 | NC_005045.1 | + | 35989 | 1.1 | 0.000336 |
Target: 5'- cGUCGAGUGCUUCCUGUGCCAGGCGACg -3' miRNA: 3'- -CAGCUCACGAAGGACACGGUCCGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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