Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22287 | 3' | -58.1 | NC_005045.1 | + | 35498 | 0.66 | 0.548559 |
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Target: 5'- uCGccGGugGCGgcuGCGGGGucgucGCCGGu -3' miRNA: 3'- uGCucCCugUGCaauCGCCCC-----UGGCC- -5' |
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| 22287 | 3' | -58.1 | NC_005045.1 | + | 12801 | 0.66 | 0.506807 |
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Target: 5'- -gGAGguGGugAUGUUGGCGGcccugucgaaGGAUCGGa -3' miRNA: 3'- ugCUC--CCugUGCAAUCGCC----------CCUGGCC- -5' |
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| 22287 | 3' | -58.1 | NC_005045.1 | + | 33603 | 0.66 | 0.506807 |
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Target: 5'- aACGAGcaGGucACGCGggAGCugcugcgccGGGACCGGg -3' miRNA: 3'- -UGCUC--CC--UGUGCaaUCGc--------CCCUGGCC- -5' |
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| 22287 | 3' | -58.1 | NC_005045.1 | + | 31833 | 0.67 | 0.466468 |
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Target: 5'- gACGAGGGuggACugGUUccagaguGCGGaagcacggucaaGGGCCGGc -3' miRNA: 3'- -UGCUCCC---UGugCAAu------CGCC------------CCUGGCC- -5' |
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| 22287 | 3' | -58.1 | NC_005045.1 | + | 19530 | 0.67 | 0.437332 |
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Target: 5'- uGCGAuGG-CGCag-AGCGGGGACuCGGc -3' miRNA: 3'- -UGCUcCCuGUGcaaUCGCCCCUG-GCC- -5' |
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| 22287 | 3' | -58.1 | NC_005045.1 | + | 36980 | 1.1 | 0.000373 |
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Target: 5'- cACGAGGGACACGUUAGCGGGGACCGGg -3' miRNA: 3'- -UGCUCCCUGUGCAAUCGCCCCUGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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