Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22289 | 5' | -50.4 | NC_005045.1 | + | 32238 | 0.66 | 0.892351 |
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Target: 5'- uGGCG-UGGGUgcucugCUGGGgucuGGUGCGGg- -3' miRNA: 3'- -CCGCuAUUCAa-----GACCUu---CCAUGCCgc -5' |
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| 22289 | 5' | -50.4 | NC_005045.1 | + | 8963 | 0.66 | 0.892351 |
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Target: 5'- cGGCGGUGGuGUUUUGGGAcugAUGGCc -3' miRNA: 3'- -CCGCUAUU-CAAGACCUUccaUGCCGc -5' |
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| 22289 | 5' | -50.4 | NC_005045.1 | + | 36870 | 0.66 | 0.892351 |
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Target: 5'- cGGUGAUcAGGUUUUuGAagugcAGGUGCuGGCGg -3' miRNA: 3'- -CCGCUA-UUCAAGAcCU-----UCCAUG-CCGC- -5' |
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| 22289 | 5' | -50.4 | NC_005045.1 | + | 33115 | 0.67 | 0.876757 |
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Target: 5'- aGGCGGUucaGGGUccUCUGGAaucGGGUAUccgucaGGCc -3' miRNA: 3'- -CCGCUA---UUCA--AGACCU---UCCAUG------CCGc -5' |
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| 22289 | 5' | -50.4 | NC_005045.1 | + | 4891 | 0.69 | 0.751812 |
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Target: 5'- cGGCGAcGAGg-CUGGAcAGGcgcuCGGCGg -3' miRNA: 3'- -CCGCUaUUCaaGACCU-UCCau--GCCGC- -5' |
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| 22289 | 5' | -50.4 | NC_005045.1 | + | 4606 | 0.7 | 0.696275 |
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Target: 5'- cGGCGGUGAGUUCaccuGGGUccccuuguCGGCGa -3' miRNA: 3'- -CCGCUAUUCAAGaccuUCCAu-------GCCGC- -5' |
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| 22289 | 5' | -50.4 | NC_005045.1 | + | 41602 | 0.72 | 0.615999 |
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Target: 5'- cGGCGGU-GGUUCcGuGAAGGUgaccauagccaACGGCGc -3' miRNA: 3'- -CCGCUAuUCAAGaC-CUUCCA-----------UGCCGC- -5' |
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| 22289 | 5' | -50.4 | NC_005045.1 | + | 37337 | 1.13 | 0.00142 |
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Target: 5'- gGGCGAUAAGUUCUGGAAGGUACGGCGg -3' miRNA: 3'- -CCGCUAUUCAAGACCUUCCAUGCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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