Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2229 | 5' | -60.6 | NC_001405.1 | + | 3942 | 0.66 | 0.282285 |
Target: 5'- gUCGUuucuCAGCAGCUguuggaUCUGCGC-CAGCAg -3' miRNA: 3'- aAGCG----GUCGUUGG------AGGCGCGcGUCGU- -5' |
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2229 | 5' | -60.6 | NC_001405.1 | + | 12207 | 0.66 | 0.275111 |
Target: 5'- -gCcCCAGCuGCCUCCaggGCGCGuCGGCu -3' miRNA: 3'- aaGcGGUCGuUGGAGG---CGCGC-GUCGu -5' |
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2229 | 5' | -60.6 | NC_001405.1 | + | 33276 | 0.66 | 0.268083 |
Target: 5'- -gCGgCGGCAGCaguuuaUUCGCGCGCugcuGCAg -3' miRNA: 3'- aaGCgGUCGUUGg-----AGGCGCGCGu---CGU- -5' |
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2229 | 5' | -60.6 | NC_001405.1 | + | 5463 | 0.66 | 0.268083 |
Target: 5'- -cCG-CGGCGuggcCCUUgGCGCGCAGCu -3' miRNA: 3'- aaGCgGUCGUu---GGAGgCGCGCGUCGu -5' |
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2229 | 5' | -60.6 | NC_001405.1 | + | 22654 | 0.66 | 0.261202 |
Target: 5'- gUCGCaguuGgGGCCUCCGCccuGCGCGcGCGa -3' miRNA: 3'- aAGCGgu--CgUUGGAGGCG---CGCGU-CGU- -5' |
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2229 | 5' | -60.6 | NC_001405.1 | + | 12664 | 0.67 | 0.254464 |
Target: 5'- --gGCCGuggcGCAGCgUgagCGCGCGCAGCAg -3' miRNA: 3'- aagCGGU----CGUUGgAg--GCGCGCGUCGU- -5' |
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2229 | 5' | -60.6 | NC_001405.1 | + | 23214 | 0.67 | 0.241419 |
Target: 5'- cUCGCCuuCGAUCUCa--GCGCAGCGg -3' miRNA: 3'- aAGCGGucGUUGGAGgcgCGCGUCGU- -5' |
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2229 | 5' | -60.6 | NC_001405.1 | + | 16402 | 0.67 | 0.235107 |
Target: 5'- cUCgGUUAGCGGCCUgCGCGUGCccguGCGc -3' miRNA: 3'- aAG-CGGUCGUUGGAgGCGCGCGu---CGU- -5' |
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2229 | 5' | -60.6 | NC_001405.1 | + | 11482 | 0.67 | 0.228935 |
Target: 5'- -gCGUCgGGCucaaaguCCUCCuCGCGCAGCAa -3' miRNA: 3'- aaGCGG-UCGuu-----GGAGGcGCGCGUCGU- -5' |
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2229 | 5' | -60.6 | NC_001405.1 | + | 14895 | 0.67 | 0.228935 |
Target: 5'- -cCGCCugAGCAACUuguugggcaUCCGCaaGCGGCAa -3' miRNA: 3'- aaGCGG--UCGUUGG---------AGGCGcgCGUCGU- -5' |
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2229 | 5' | -60.6 | NC_001405.1 | + | 6620 | 0.68 | 0.200102 |
Target: 5'- -gCGCCuGCuGCCaUgCGCGgGCGGCAa -3' miRNA: 3'- aaGCGGuCGuUGG-AgGCGCgCGUCGU- -5' |
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2229 | 5' | -60.6 | NC_001405.1 | + | 26438 | 0.69 | 0.178867 |
Target: 5'- aUCGCUA-CAACCUCCGCuccucagGCGCcgccGGCAc -3' miRNA: 3'- aAGCGGUcGUUGGAGGCG-------CGCG----UCGU- -5' |
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2229 | 5' | -60.6 | NC_001405.1 | + | 15791 | 0.69 | 0.174 |
Target: 5'- cUCGCUGGacuCCUCCGaugcuguUGCGCAGCGg -3' miRNA: 3'- aAGCGGUCguuGGAGGC-------GCGCGUCGU- -5' |
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2229 | 5' | -60.6 | NC_001405.1 | + | 23989 | 0.69 | 0.169722 |
Target: 5'- gUCGCC-GCcGCCUccCCGCGCGCccccaaaaAGCGg -3' miRNA: 3'- aAGCGGuCGuUGGA--GGCGCGCG--------UCGU- -5' |
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2229 | 5' | -60.6 | NC_001405.1 | + | 3759 | 0.69 | 0.169722 |
Target: 5'- -aCGCCGuuggagacuGCAGCCUCCGC-CGCcGCu -3' miRNA: 3'- aaGCGGU---------CGUUGGAGGCGcGCGuCGu -5' |
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2229 | 5' | -60.6 | NC_001405.1 | + | 4154 | 0.7 | 0.151825 |
Target: 5'- -cCGCUGGUcccgGGCCUaCCGCGCGC-GCAa -3' miRNA: 3'- aaGCGGUCG----UUGGA-GGCGCGCGuCGU- -5' |
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2229 | 5' | -60.6 | NC_001405.1 | + | 8703 | 0.7 | 0.147627 |
Target: 5'- ---aCCAGCu-CCUgcCCGCGCGCGGCGc -3' miRNA: 3'- aagcGGUCGuuGGA--GGCGCGCGUCGU- -5' |
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2229 | 5' | -60.6 | NC_001405.1 | + | 10881 | 0.71 | 0.121089 |
Target: 5'- uUUCGCuuucCAGCcuAACCgcuuacgCCGCGCGCGGCc -3' miRNA: 3'- -AAGCG----GUCG--UUGGa------GGCGCGCGUCGu -5' |
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2229 | 5' | -60.6 | NC_001405.1 | + | 12557 | 0.72 | 0.107985 |
Target: 5'- aUCGCCAGCAcCUUCuCGUGCGUGGgGu -3' miRNA: 3'- aAGCGGUCGUuGGAG-GCGCGCGUCgU- -5' |
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2229 | 5' | -60.6 | NC_001405.1 | + | 10787 | 0.74 | 0.074038 |
Target: 5'- -gCGCUAGCuuuuuuGGCCacuggCCGCGCGCGGCGu -3' miRNA: 3'- aaGCGGUCG------UUGGa----GGCGCGCGUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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