Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22290 | 3' | -62.8 | NC_005045.1 | + | 18590 | 0.66 | 0.282589 |
Target: 5'- gAACCCAa-CCGcUUCACaGGGCGGGGc- -3' miRNA: 3'- -UUGGGUgcGGC-AGGUG-CCCGUCCCac -5' |
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22290 | 3' | -62.8 | NC_005045.1 | + | 40343 | 0.66 | 0.282589 |
Target: 5'- cACCCuCGCCGUCaC-CGGcGUGGGGa- -3' miRNA: 3'- uUGGGuGCGGCAG-GuGCC-CGUCCCac -5' |
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22290 | 3' | -62.8 | NC_005045.1 | + | 39699 | 0.66 | 0.275692 |
Target: 5'- cAGCUCGuCGCCGccgUCGCcGGCAGGGUc -3' miRNA: 3'- -UUGGGU-GCGGCa--GGUGcCCGUCCCAc -5' |
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22290 | 3' | -62.8 | NC_005045.1 | + | 32552 | 0.66 | 0.258388 |
Target: 5'- aGACCCACGCCGaggugccuaagccgcUCCA-GGGCAucgccgccaaGGUGg -3' miRNA: 3'- -UUGGGUGCGGC---------------AGGUgCCCGUc---------CCAC- -5' |
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22290 | 3' | -62.8 | NC_005045.1 | + | 40409 | 0.66 | 0.255806 |
Target: 5'- -cCCCACGCCGgUgACGGcG-AGGGUGc -3' miRNA: 3'- uuGGGUGCGGCaGgUGCC-CgUCCCAC- -5' |
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22290 | 3' | -62.8 | NC_005045.1 | + | 13029 | 0.67 | 0.248815 |
Target: 5'- gAGCCUgcaucauGCGaCCGUgCCACGGGUgguuGGGGUc -3' miRNA: 3'- -UUGGG-------UGC-GGCA-GGUGCCCG----UCCCAc -5' |
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22290 | 3' | -62.8 | NC_005045.1 | + | 27360 | 0.67 | 0.243213 |
Target: 5'- uGGCCCcguCGCCGUCCACGGaCAGc--- -3' miRNA: 3'- -UUGGGu--GCGGCAGGUGCCcGUCccac -5' |
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22290 | 3' | -62.8 | NC_005045.1 | + | 41578 | 0.67 | 0.219576 |
Target: 5'- cGCCCuCGCCGcCCA-GGGCAGuGGcUGc -3' miRNA: 3'- uUGGGuGCGGCaGGUgCCCGUC-CC-AC- -5' |
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22290 | 3' | -62.8 | NC_005045.1 | + | 16761 | 0.68 | 0.20851 |
Target: 5'- gGACCCugGCCGaccacguugcCCACGGcGcCGGGGa- -3' miRNA: 3'- -UUGGGugCGGCa---------GGUGCC-C-GUCCCac -5' |
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22290 | 3' | -62.8 | NC_005045.1 | + | 35833 | 0.68 | 0.192815 |
Target: 5'- uGCCCugGCUgaacugcguGUCCGggaccUGGGCAGGcGUGu -3' miRNA: 3'- uUGGGugCGG---------CAGGU-----GCCCGUCC-CAC- -5' |
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22290 | 3' | -62.8 | NC_005045.1 | + | 5234 | 0.71 | 0.112519 |
Target: 5'- uGCCCugGCCGUgugCCagcaGCGGGCAGaGGa- -3' miRNA: 3'- uUGGGugCGGCA---GG----UGCCCGUC-CCac -5' |
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22290 | 3' | -62.8 | NC_005045.1 | + | 10213 | 0.77 | 0.044468 |
Target: 5'- -cUCCAgGCCGUCCA-GGGCAGGGa- -3' miRNA: 3'- uuGGGUgCGGCAGGUgCCCGUCCCac -5' |
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22290 | 3' | -62.8 | NC_005045.1 | + | 41654 | 0.91 | 0.003084 |
Target: 5'- gAACCaC-CGCCGUCCACGGGCAGGGUGu -3' miRNA: 3'- -UUGG-GuGCGGCAGGUGCCCGUCCCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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