miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22290 5' -57.1 NC_005045.1 + 41619 1.06 0.000643
Target:  5'- cUUCACCUUGUAGGCGGUGAGCGGGUCc -3'
miRNA:   3'- -AAGUGGAACAUCCGCCACUCGCCCAG- -5'
22290 5' -57.1 NC_005045.1 + 4622 0.77 0.09557
Target:  5'- cUUCGCCUUGgucacucGGCGGUGAGUucaccuGGGUCc -3'
miRNA:   3'- -AAGUGGAACau-----CCGCCACUCG------CCCAG- -5'
22290 5' -57.1 NC_005045.1 + 25058 0.67 0.417856
Target:  5'- -gCGCCacuUGGGCGGUGAucaGGGUCu -3'
miRNA:   3'- aaGUGGaacAUCCGCCACUcg-CCCAG- -5'
22290 5' -57.1 NC_005045.1 + 42000 0.66 0.520413
Target:  5'- gUUgGCUggUGUGGGCGG-GAGUaguuacuccGGGUCc -3'
miRNA:   3'- -AAgUGGa-ACAUCCGCCaCUCG---------CCCAG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.