Results 1 - 11 of 11 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22292 | 5' | -51.1 | NC_005045.1 | + | 37334 | 0.66 | 0.842826 |
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Target: 5'- gUGGCGCGCUAUCAGuGCGcCGGa-- -3' miRNA: 3'- aACUGUGUGAUGGUUcCGUaGCCauc -5' |
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| 22292 | 5' | -51.1 | NC_005045.1 | + | 35770 | 0.66 | 0.813918 |
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Target: 5'- -gGACACGCaguucaGCCAGGGCAUCc---- -3' miRNA: 3'- aaCUGUGUGa-----UGGUUCCGUAGccauc -5' |
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| 22292 | 5' | -51.1 | NC_005045.1 | + | 41583 | 0.66 | 0.803832 |
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Target: 5'- gUGACACcCUGCCcguGGaCggCGGUGGu -3' miRNA: 3'- aACUGUGuGAUGGuu-CC-GuaGCCAUC- -5' |
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| 22292 | 5' | -51.1 | NC_005045.1 | + | 2660 | 0.68 | 0.716914 |
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Target: 5'- cUUGAaGCugUACCAcGGGCuGUUGGUGGc -3' miRNA: 3'- -AACUgUGugAUGGU-UCCG-UAGCCAUC- -5' |
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| 22292 | 5' | -51.1 | NC_005045.1 | + | 5294 | 0.68 | 0.716914 |
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Target: 5'- cUGGCACACgGCCAGGGCAg------ -3' miRNA: 3'- aACUGUGUGaUGGUUCCGUagccauc -5' |
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| 22292 | 5' | -51.1 | NC_005045.1 | + | 31558 | 0.68 | 0.705475 |
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Target: 5'- -cGGCGCugUACCAAucgccccgaucuGGC-UCGGUGu -3' miRNA: 3'- aaCUGUGugAUGGUU------------CCGuAGCCAUc -5' |
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| 22292 | 5' | -51.1 | NC_005045.1 | + | 23346 | 0.69 | 0.670725 |
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Target: 5'- aUGACgccGCACUACCAGccGGUggCGGUGc -3' miRNA: 3'- aACUG---UGUGAUGGUU--CCGuaGCCAUc -5' |
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| 22292 | 5' | -51.1 | NC_005045.1 | + | 1135 | 0.69 | 0.670725 |
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Target: 5'- cUGACACGgUG-CAGGGCAcgCGGUAc -3' miRNA: 3'- aACUGUGUgAUgGUUCCGUa-GCCAUc -5' |
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| 22292 | 5' | -51.1 | NC_005045.1 | + | 24037 | 0.69 | 0.670725 |
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Target: 5'- -gGACAgaGCUGCCAGGGCA--GGUGc -3' miRNA: 3'- aaCUGUg-UGAUGGUUCCGUagCCAUc -5' |
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| 22292 | 5' | -51.1 | NC_005045.1 | + | 15253 | 0.71 | 0.55404 |
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Target: 5'- cUGGCACACUGCaCAGGGaCA-CGGg-- -3' miRNA: 3'- aACUGUGUGAUG-GUUCC-GUaGCCauc -5' |
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| 22292 | 5' | -51.1 | NC_005045.1 | + | 981 | 0.94 | 0.018567 |
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Target: 5'- gUUGACACACUACCAAGG-AUCGGUAGa -3' miRNA: 3'- -AACUGUGUGAUGGUUCCgUAGCCAUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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