Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22297 | 5' | -53.4 | NC_005045.1 | + | 13894 | 0.67 | 0.642977 |
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Target: 5'- cCAGUGCUGCCgCGcugccguccgGGUgGUCCAcGAa -3' miRNA: 3'- -GUCACGACGG-GCa---------CCAaUAGGUaCUg -5' |
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| 22297 | 5' | -53.4 | NC_005045.1 | + | 3867 | 0.68 | 0.608492 |
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Target: 5'- -uGUGCUggcaacuucagGCCCGUGGcacauccgGUCCGUGGu -3' miRNA: 3'- guCACGA-----------CGGGCACCaa------UAGGUACUg -5' |
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| 22297 | 5' | -53.4 | NC_005045.1 | + | 26813 | 0.69 | 0.518208 |
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Target: 5'- uGGUGgaGCCgGUGG----CCAUGACa -3' miRNA: 3'- gUCACgaCGGgCACCaauaGGUACUG- -5' |
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| 22297 | 5' | -53.4 | NC_005045.1 | + | 41580 | 0.69 | 0.513816 |
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Target: 5'- aCGGUGacaccCUGCCCGUGGacggcggugguUCCGUGAa -3' miRNA: 3'- -GUCAC-----GACGGGCACCaau--------AGGUACUg -5' |
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| 22297 | 5' | -53.4 | NC_005045.1 | + | 26388 | 0.8 | 0.109037 |
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Target: 5'- aGGUGgUGCCCGUGGcgUUAUCCuUGACc -3' miRNA: 3'- gUCACgACGGGCACC--AAUAGGuACUG- -5' |
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| 22297 | 5' | -53.4 | NC_005045.1 | + | 4436 | 1.1 | 0.000776 |
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Target: 5'- gCAGUGCUGCCCGUGGUUAUCCAUGACg -3' miRNA: 3'- -GUCACGACGGGCACCAAUAGGUACUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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