Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22298 | 5' | -62.2 | NC_005045.1 | + | 10208 | 0.66 | 0.334162 |
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Target: 5'- --gGCCGUCCAGggcaGggaagCGGC-GCCCGc -3' miRNA: 3'- gcgCGGCAGGUCag--Ca----GCCGuCGGGU- -5' |
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| 22298 | 5' | -62.2 | NC_005045.1 | + | 7753 | 0.66 | 0.326367 |
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Target: 5'- gCGCGCCcaaUCCAGcccgCGUCGGUagggaagauaAGCCg- -3' miRNA: 3'- -GCGCGGc--AGGUCa---GCAGCCG----------UCGGgu -5' |
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| 22298 | 5' | -62.2 | NC_005045.1 | + | 7953 | 0.66 | 0.318707 |
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Target: 5'- -uCGCCG-CCAGcCuGUCGcGCAGCCUu -3' miRNA: 3'- gcGCGGCaGGUCaG-CAGC-CGUCGGGu -5' |
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| 22298 | 5' | -62.2 | NC_005045.1 | + | 10758 | 0.67 | 0.275593 |
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Target: 5'- gGUGCCGgaaccaucuUCCAGcUCcacaCGGUAGCCCAc -3' miRNA: 3'- gCGCGGC---------AGGUC-AGca--GCCGUCGGGU- -5' |
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| 22298 | 5' | -62.2 | NC_005045.1 | + | 29683 | 0.68 | 0.21984 |
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Target: 5'- -aUGCUGUCCAGgaagccggCGUCGcGCAGaCCCu -3' miRNA: 3'- gcGCGGCAGGUCa-------GCAGC-CGUC-GGGu -5' |
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| 22298 | 5' | -62.2 | NC_005045.1 | + | 13600 | 0.69 | 0.208836 |
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Target: 5'- aGCGUCGgCCAuGaUGUCGcGCAGCCCu -3' miRNA: 3'- gCGCGGCaGGU-CaGCAGC-CGUCGGGu -5' |
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| 22298 | 5' | -62.2 | NC_005045.1 | + | 39250 | 0.72 | 0.119537 |
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Target: 5'- aCGCGCCugcucGUCCAGg-GUCGGgaacCGGCCCGg -3' miRNA: 3'- -GCGCGG-----CAGGUCagCAGCC----GUCGGGU- -5' |
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| 22298 | 5' | -62.2 | NC_005045.1 | + | 39579 | 0.72 | 0.110136 |
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Target: 5'- aGCGUCGaacUUgAGcuugaagcgcUCGUCGGCAGCCCAg -3' miRNA: 3'- gCGCGGC---AGgUC----------AGCAGCCGUCGGGU- -5' |
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| 22298 | 5' | -62.2 | NC_005045.1 | + | 5001 | 1.08 | 0.000191 |
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Target: 5'- cCGCGCCGUCCAGUCGUCGGCAGCCCAg -3' miRNA: 3'- -GCGCGGCAGGUCAGCAGCCGUCGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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