Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22299 | 3' | -52.7 | NC_005045.1 | + | 20894 | 0.72 | 0.460862 |
Target: 5'- gCGAGUccacgcaAAGGCCgGCGCcccauccgaacUGAUGGGCCu -3' miRNA: 3'- -GCUCA-------UUCCGGgCGUGc----------AUUAUCCGG- -5' |
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22299 | 3' | -52.7 | NC_005045.1 | + | 27336 | 0.73 | 0.402351 |
Target: 5'- uGGGUuucauGGGCCUGCAUGaGAU-GGCCc -3' miRNA: 3'- gCUCAu----UCCGGGCGUGCaUUAuCCGG- -5' |
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22299 | 3' | -52.7 | NC_005045.1 | + | 5060 | 1.12 | 0.000908 |
Target: 5'- gCGAGUAAGGCCCGCACGUAAUAGGCCc -3' miRNA: 3'- -GCUCAUUCCGGGCGUGCAUUAUCCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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