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miRNA: 3'- gCCCGGAUaACGcUCGAUCGgUAUGg -5'
miRNA: 3'- gCCCGGAuaa--CGCUCGAUCGguauGG- -5'
miRNA: 3'- -GCCCGGauaacGCUCGAUCGGU-AUGG- -5'
miRNA: 3'- gCCCGGAuaaCGCUCGa-----UCGGUAuGG- -5'
miRNA: 3'- -GCCCGGAUAACGCUCGAUCGGUAUGG- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 22299 | 5' | -53.7 | NC_005045.1 | + | 20932 | 0.73 | 0.303656 |
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Target: 5'- uGGGCCUG-UGCuGGCUGGCaGUACa -3' miRNA: 3'- gCCCGGAUaACGcUCGAUCGgUAUGg -5' |
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| 22299 | 5' | -53.7 | NC_005045.1 | + | 40127 | 0.75 | 0.246097 |
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Target: 5'- aGGGCCUuguccGCGAGCUAGCac--CCa -3' miRNA: 3'- gCCCGGAuaa--CGCUCGAUCGguauGG- -5' |
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| 22299 | 5' | -53.7 | NC_005045.1 | + | 6959 | 0.76 | 0.214918 |
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Target: 5'- aCGGGCCgc---CGAGCUGGCUGgUGCCg -3' miRNA: 3'- -GCCCGGauaacGCUCGAUCGGU-AUGG- -5' |
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| 22299 | 5' | -53.7 | NC_005045.1 | + | 20050 | 0.77 | 0.187189 |
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Target: 5'- aGGGCCUua-GCGAGCcgcuggAGCCAUuCCa -3' miRNA: 3'- gCCCGGAuaaCGCUCGa-----UCGGUAuGG- -5' |
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| 22299 | 5' | -53.7 | NC_005045.1 | + | 5094 | 1.13 | 0.000479 |
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Target: 5'- cCGGGCCUAUUGCGAGCUAGCCAUACCa -3' miRNA: 3'- -GCCCGGAUAACGCUCGAUCGGUAUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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