Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
223 | 3' | -52.9 | AC_000008.1 | + | 11132 | 0.66 | 0.729841 |
Target: 5'- aCC-GcgUCAGGAGGggCGACauCCGCGg -3' miRNA: 3'- -GGuCuaAGUCCUCUa-GUUGgcGGCGC- -5' |
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223 | 3' | -52.9 | AC_000008.1 | + | 18783 | 0.66 | 0.718795 |
Target: 5'- gCCAGA----GGAGcugcUgAGCCGCCGCGc -3' miRNA: 3'- -GGUCUaaguCCUCu---AgUUGGCGGCGC- -5' |
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223 | 3' | -52.9 | AC_000008.1 | + | 18065 | 0.67 | 0.707655 |
Target: 5'- aUCAGuggaauaAGGAGGggCAggauACCGCCGCGc -3' miRNA: 3'- -GGUCuaag---UCCUCUa-GU----UGGCGGCGC- -5' |
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223 | 3' | -52.9 | AC_000008.1 | + | 8635 | 0.67 | 0.685149 |
Target: 5'- -aGGGggCAGGGGcacGUCGgcGCCGCgCGCGg -3' miRNA: 3'- ggUCUaaGUCCUC---UAGU--UGGCG-GCGC- -5' |
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223 | 3' | -52.9 | AC_000008.1 | + | 17265 | 0.67 | 0.673807 |
Target: 5'- gCGGAUgccgcggugCAGGcGGUCGcuGCgGCCGCGu -3' miRNA: 3'- gGUCUAa--------GUCCuCUAGU--UGgCGGCGC- -5' |
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223 | 3' | -52.9 | AC_000008.1 | + | 26088 | 0.67 | 0.67267 |
Target: 5'- cCCAGuccggCgAGGAGcUCAacccaauccccccGCCGCCGCa -3' miRNA: 3'- -GGUCuaa--G-UCCUCuAGU-------------UGGCGGCGc -5' |
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223 | 3' | -52.9 | AC_000008.1 | + | 26797 | 0.68 | 0.651009 |
Target: 5'- gCAGcagCAGGAGGagGAgCGCUGCGu -3' miRNA: 3'- gGUCuaaGUCCUCUagUUgGCGGCGC- -5' |
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223 | 3' | -52.9 | AC_000008.1 | + | 27050 | 0.69 | 0.582516 |
Target: 5'- cUCAGGagacgugCAGGAGAUUuugcgccaGGCCGCCGUc -3' miRNA: 3'- -GGUCUaa-----GUCCUCUAG--------UUGGCGGCGc -5' |
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223 | 3' | -52.9 | AC_000008.1 | + | 21648 | 0.7 | 0.498237 |
Target: 5'- gCGGGUcCAGGGGAgcaUCGaagggagaacccagcGCCGCCGCc -3' miRNA: 3'- gGUCUAaGUCCUCU---AGU---------------UGGCGGCGc -5' |
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223 | 3' | -52.9 | AC_000008.1 | + | 16315 | 0.78 | 0.178242 |
Target: 5'- cCCAGGUcCAGGcGAcgagCGGCCGCCGCa -3' miRNA: 3'- -GGUCUAaGUCCuCUa---GUUGGCGGCGc -5' |
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223 | 3' | -52.9 | AC_000008.1 | + | 27166 | 1.11 | 0.000876 |
Target: 5'- gCCAGAUUCAGGAGAUCAACCGCCGCGu -3' miRNA: 3'- -GGUCUAAGUCCUCUAGUUGGCGGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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