Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 223 | 5' | -58.4 | AC_000008.1 | + | 24467 | 0.71 | 0.157287 |
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Target: 5'- cUCGCgCAGCAACCgcuCGCGauucaggccaugcCGCAGCu -3' miRNA: 3'- aAGCG-GUCGUUGGau-GCGC-------------GCGUCGu -5' |
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| 223 | 5' | -58.4 | AC_000008.1 | + | 5473 | 0.72 | 0.153319 |
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Target: 5'- -cCG-CGGCGuggcCCUugGCGCGCAGCu -3' miRNA: 3'- aaGCgGUCGUu---GGAugCGCGCGUCGu -5' |
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| 223 | 5' | -58.4 | AC_000008.1 | + | 23372 | 0.72 | 0.136764 |
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Target: 5'- aUCGCCAGC-ACCUucuCGUGCGUGGgGu -3' miRNA: 3'- aAGCGGUCGuUGGAu--GCGCGCGUCgU- -5' |
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| 223 | 5' | -58.4 | AC_000008.1 | + | 12674 | 0.74 | 0.105365 |
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Target: 5'- --gGCCGuggcGCAGCgUgaGCGCGCGCAGCAg -3' miRNA: 3'- aagCGGU----CGUUGgA--UGCGCGCGUCGU- -5' |
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| 223 | 5' | -58.4 | AC_000008.1 | + | 16407 | 0.74 | 0.102327 |
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Target: 5'- cUCgGUUAGCGGCCUGCGCGUGCccguGCGc -3' miRNA: 3'- aAG-CGGUCGUUGGAUGCGCGCGu---CGU- -5' |
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| 223 | 5' | -58.4 | AC_000008.1 | + | 6630 | 0.75 | 0.085766 |
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Target: 5'- -gCGCCuGCuGCCaUGCGCGgGCGGCAa -3' miRNA: 3'- aaGCGGuCGuUGG-AUGCGCgCGUCGU- -5' |
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| 223 | 5' | -58.4 | AC_000008.1 | + | 27200 | 1.05 | 0.000365 |
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Target: 5'- gUUCGCCAGCAACCUACGCGCGCAGCAc -3' miRNA: 3'- -AAGCGGUCGUUGGAUGCGCGCGUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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