Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2230 | 3' | -53.4 | NC_001405.1 | + | 9990 | 0.66 | 0.669636 |
Target: 5'- uCGCuacGGcgGCGGCGgaguuuGGCCGUAggUGGCg -3' miRNA: 3'- uGCGu--CCa-CGUCGU------CCGGUAUa-AUCG- -5' |
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2230 | 3' | -53.4 | NC_001405.1 | + | 13956 | 0.66 | 0.669636 |
Target: 5'- gACGaCGGGUG-GGCGGGCgCGggccgGGCa -3' miRNA: 3'- -UGC-GUCCACgUCGUCCG-GUauaa-UCG- -5' |
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2230 | 3' | -53.4 | NC_001405.1 | + | 26075 | 0.66 | 0.668471 |
Target: 5'- gACGCAGGcgGUaagcuccgcauuuGGCGGGCgGgAUUGGUc -3' miRNA: 3'- -UGCGUCCa-CG-------------UCGUCCGgUaUAAUCG- -5' |
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2230 | 3' | -53.4 | NC_001405.1 | + | 12150 | 0.66 | 0.657971 |
Target: 5'- cGCGCccuGGagGCAGCugGGGCCGgaccugGGCu -3' miRNA: 3'- -UGCGu--CCa-CGUCG--UCCGGUauaa--UCG- -5' |
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2230 | 3' | -53.4 | NC_001405.1 | + | 3847 | 0.66 | 0.657971 |
Target: 5'- gGCGguGGcUGCAGC-GGCUgaAgcGGCg -3' miRNA: 3'- -UGCguCC-ACGUCGuCCGGuaUaaUCG- -5' |
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2230 | 3' | -53.4 | NC_001405.1 | + | 12670 | 0.66 | 0.634573 |
Target: 5'- gGCGCAGcGUgagcgcgcgcaGCAGCAGGgCAaccugGGCu -3' miRNA: 3'- -UGCGUC-CA-----------CGUCGUCCgGUauaa-UCG- -5' |
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2230 | 3' | -53.4 | NC_001405.1 | + | 21003 | 0.66 | 0.622864 |
Target: 5'- -aGUAGGUGUAGUaaGGGUCGU---AGCc -3' miRNA: 3'- ugCGUCCACGUCG--UCCGGUAuaaUCG- -5' |
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2230 | 3' | -53.4 | NC_001405.1 | + | 16474 | 0.67 | 0.599484 |
Target: 5'- -gGCGGGUGCGcacgggcacgcGCAGGCCGcuaaccgAGUc -3' miRNA: 3'- ugCGUCCACGU-----------CGUCCGGUauaa---UCG- -5' |
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2230 | 3' | -53.4 | NC_001405.1 | + | 9478 | 0.67 | 0.598318 |
Target: 5'- gGCGCAuggucucGGUGaCGGCGcGGCCGUucucgcgggGGCg -3' miRNA: 3'- -UGCGU-------CCAC-GUCGU-CCGGUAuaa------UCG- -5' |
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2230 | 3' | -53.4 | NC_001405.1 | + | 24231 | 0.67 | 0.580867 |
Target: 5'- aGCGgGGGUGCcucgacggggaagguGGUAGGCgc-GUUGGCg -3' miRNA: 3'- -UGCgUCCACG---------------UCGUCCGguaUAAUCG- -5' |
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2230 | 3' | -53.4 | NC_001405.1 | + | 18504 | 0.67 | 0.576231 |
Target: 5'- -gGguGGUG-GGCAGGCCuugcuUUAGUg -3' miRNA: 3'- ugCguCCACgUCGUCCGGuau--AAUCG- -5' |
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2230 | 3' | -53.4 | NC_001405.1 | + | 8919 | 0.67 | 0.561223 |
Target: 5'- cACGguGGcgGCGaggucguuggagauGCGGGCCAUG--AGCu -3' miRNA: 3'- -UGCguCCa-CGU--------------CGUCCGGUAUaaUCG- -5' |
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2230 | 3' | -53.4 | NC_001405.1 | + | 22724 | 0.67 | 0.553185 |
Target: 5'- aGCGcCGGGUGguGCAcgcuGGCCAgcac-GCu -3' miRNA: 3'- -UGC-GUCCACguCGU----CCGGUauaauCG- -5' |
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2230 | 3' | -53.4 | NC_001405.1 | + | 22716 | 0.67 | 0.553185 |
Target: 5'- cGCGCAGG-GCGG-AGGCCccaacuGCg -3' miRNA: 3'- -UGCGUCCaCGUCgUCCGGuauaauCG- -5' |
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2230 | 3' | -53.4 | NC_001405.1 | + | 23688 | 0.68 | 0.531553 |
Target: 5'- aGCGCAcGGUGCGGCGGcugaaugaagacgacCCAg--UGGCg -3' miRNA: 3'- -UGCGU-CCACGUCGUCc--------------GGUauaAUCG- -5' |
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2230 | 3' | -53.4 | NC_001405.1 | + | 15824 | 0.68 | 0.530423 |
Target: 5'- -gGCAGGUGCGGCGucuGGCgucaGUAaUGGUc -3' miRNA: 3'- ugCGUCCACGUCGU---CCGg---UAUaAUCG- -5' |
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2230 | 3' | -53.4 | NC_001405.1 | + | 26780 | 0.68 | 0.530423 |
Target: 5'- cCGCcGGUGCAGUagGGGCUGUAgagaugacggUAGUa -3' miRNA: 3'- uGCGuCCACGUCG--UCCGGUAUa---------AUCG- -5' |
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2230 | 3' | -53.4 | NC_001405.1 | + | 27459 | 0.68 | 0.508011 |
Target: 5'- cAC-CAGG-GCAGCGGGCCAa------ -3' miRNA: 3'- -UGcGUCCaCGUCGUCCGGUauaaucg -5' |
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2230 | 3' | -53.4 | NC_001405.1 | + | 17718 | 0.68 | 0.496955 |
Target: 5'- aACGgAGGcgGCAGgugaGGGCCAUAUcuGCa -3' miRNA: 3'- -UGCgUCCa-CGUCg---UCCGGUAUAauCG- -5' |
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2230 | 3' | -53.4 | NC_001405.1 | + | 8520 | 0.69 | 0.46447 |
Target: 5'- cGCGCGGcGgGCGGUGGGCCGcGggGGUg -3' miRNA: 3'- -UGCGUC-CaCGUCGUCCGGUaUaaUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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