Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2230 | 5' | -61.2 | NC_001405.1 | + | 12121 | 0.66 | 0.304658 |
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Target: 5'- gCGCUGACCUGcGCUggGCcccaAGCCGa -3' miRNA: 3'- aGCGGCUGGAC-CGGgaCGaag-UCGGC- -5' |
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| 2230 | 5' | -61.2 | NC_001405.1 | + | 12187 | 0.66 | 0.304658 |
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Target: 5'- gCGUCGGCUuggGGCCCaGCgcaggUCAGCg- -3' miRNA: 3'- aGCGGCUGGa--CCGGGaCGa----AGUCGgc -5' |
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| 2230 | 5' | -61.2 | NC_001405.1 | + | 2217 | 0.66 | 0.289887 |
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Target: 5'- cCGCCGccGCCUGGCuuccuCCUGCUguuGCUc -3' miRNA: 3'- aGCGGC--UGGACCG-----GGACGAaguCGGc -5' |
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| 2230 | 5' | -61.2 | NC_001405.1 | + | 3759 | 0.66 | 0.275684 |
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Target: 5'- aCGCCGuuggagacugcaGCCUccGCCgCcGCUUCAGCCGc -3' miRNA: 3'- aGCGGC------------UGGAc-CGG-GaCGAAGUCGGC- -5' |
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| 2230 | 5' | -61.2 | NC_001405.1 | + | 34108 | 0.68 | 0.226762 |
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Target: 5'- aCGcCCGcACCUGGUuuUGCUUCAGgaaauaugacuacguCCGg -3' miRNA: 3'- aGC-GGC-UGGACCGggACGAAGUC---------------GGC- -5' |
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| 2230 | 5' | -61.2 | NC_001405.1 | + | 5398 | 0.72 | 0.104464 |
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Target: 5'- cUGCCGGuCUUcGCCCUGCgcgUCGGCCa -3' miRNA: 3'- aGCGGCU-GGAcCGGGACGa--AGUCGGc -5' |
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| 2230 | 5' | -61.2 | NC_001405.1 | + | 12607 | 0.77 | 0.048015 |
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Target: 5'- -gGCCGGCCucaucgggccggaUGGCCCUGuUUUCGGCCa -3' miRNA: 3'- agCGGCUGG-------------ACCGGGAC-GAAGUCGGc -5' |
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| 2230 | 5' | -61.2 | NC_001405.1 | + | 12565 | 0.78 | 0.040433 |
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Target: 5'- -gGCCGGCCUGGUCuacgacgcgCUGCUUCAGCgCGu -3' miRNA: 3'- agCGGCUGGACCGG---------GACGAAGUCG-GC- -5' |
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| 2230 | 5' | -61.2 | NC_001405.1 | + | 10127 | 1.09 | 0.000144 |
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Target: 5'- gUCGCCGACCUGGCCCUGCUUCAGCCGa -3' miRNA: 3'- -AGCGGCUGGACCGGGACGAAGUCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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