miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22301 3' -58.7 NC_005045.1 + 32942 0.66 0.414035
Target:  5'- cGCCGCcggUCGGCaacuGUcCGAGUUcGAGGAg -3'
miRNA:   3'- aUGGCGa--AGCCG----CA-GCUCGAcCUCCU- -5'
22301 3' -58.7 NC_005045.1 + 19590 0.67 0.386474
Target:  5'- aGCCGCUUCGGUaccccUCGGGauuccGGAGGc -3'
miRNA:   3'- aUGGCGAAGCCGc----AGCUCga---CCUCCu -5'
22301 3' -58.7 NC_005045.1 + 14163 0.68 0.319126
Target:  5'- gGCCGCUUCuGCaucuacaUCGAGCUGGgugaccacgccaAGGAa -3'
miRNA:   3'- aUGGCGAAGcCGc------AGCUCGACC------------UCCU- -5'
22301 3' -58.7 NC_005045.1 + 31878 0.78 0.067262
Target:  5'- cGCCGCaaggUCuGCGUCGAGCUGGAGc- -3'
miRNA:   3'- aUGGCGa---AGcCGCAGCUCGACCUCcu -5'
22301 3' -58.7 NC_005045.1 + 6415 1.07 0.000453
Target:  5'- aUACCGCUUCGGCGUCGAGCUGGAGGAc -3'
miRNA:   3'- -AUGGCGAAGCCGCAGCUCGACCUCCU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.