Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22303 | 5' | -48.8 | NC_005045.1 | + | 19529 | 0.67 | 0.911974 |
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Target: 5'- cCGAGGGGUAcCGaagCGGCUUcAUCgUCCa -3' miRNA: 3'- -GCUUUCCGUcGUa--GCCGAA-UAGaAGG- -5' |
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| 22303 | 5' | -48.8 | NC_005045.1 | + | 35995 | 0.67 | 0.897679 |
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Target: 5'- ----uGGCGGCGUCGaGUg---CUUCCu -3' miRNA: 3'- gcuuuCCGUCGUAGC-CGaauaGAAGG- -5' |
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| 22303 | 5' | -48.8 | NC_005045.1 | + | 32996 | 0.68 | 0.882165 |
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Target: 5'- cCGAccGGCGGCG-CGGuCUUG-CUUCa -3' miRNA: 3'- -GCUuuCCGUCGUaGCC-GAAUaGAAGg -5' |
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| 22303 | 5' | -48.8 | NC_005045.1 | + | 23535 | 0.68 | 0.856717 |
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Target: 5'- -uGAAGGcCGGCggCGGCggGaCUUCCa -3' miRNA: 3'- gcUUUCC-GUCGuaGCCGaaUaGAAGG- -5' |
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| 22303 | 5' | -48.8 | NC_005045.1 | + | 14107 | 0.69 | 0.838398 |
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Target: 5'- cCGAAAccuccaccGGCGGUggCGGCggucgcAUCUUCCc -3' miRNA: 3'- -GCUUU--------CCGUCGuaGCCGaa----UAGAAGG- -5' |
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| 22303 | 5' | -48.8 | NC_005045.1 | + | 21951 | 0.69 | 0.838398 |
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Target: 5'- cCGAgccAAGGCAGCAgucacuUCGGUaaUGUCUgugUCCc -3' miRNA: 3'- -GCU---UUCCGUCGU------AGCCGa-AUAGA---AGG- -5' |
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| 22303 | 5' | -48.8 | NC_005045.1 | + | 16302 | 0.7 | 0.777884 |
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Target: 5'- gGAGGGGCcgaAGCAUCcGCUgAUCUaUCCc -3' miRNA: 3'- gCUUUCCG---UCGUAGcCGAaUAGA-AGG- -5' |
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| 22303 | 5' | -48.8 | NC_005045.1 | + | 13605 | 0.71 | 0.733927 |
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Target: 5'- ---cGGGCAGCGUCGGCcaugaUGUCgcgcagCCc -3' miRNA: 3'- gcuuUCCGUCGUAGCCGa----AUAGaa----GG- -5' |
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| 22303 | 5' | -48.8 | NC_005045.1 | + | 7658 | 1.13 | 0.001948 |
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Target: 5'- gCGAAAGGCAGCAUCGGCUUAUCUUCCc -3' miRNA: 3'- -GCUUUCCGUCGUAGCCGAAUAGAAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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